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Microtubule dynamics in an Arabidopsis Leaf --- TIRF microscopy wasteneyslaboratory 193 views Microtubules are visualized here using the microtubule plus end marker EB1 fused to green fluorescent protein (GFP). We can achive very good contrast with minimal photoblea...
Microtubules in Arabidopsis Root Hair --- TIRF microscopy wasteneyslaboratory 315 views This root hair is growing right along the coverslip, allowing us to investigate cortical microtubule dynamics using total internal reflection fluorescence (TIRF) microscopy...
Plating Arabidopsis Seeds wasteneyslaboratory 361 views We just do 5 minutes in 70% ethanol to sterilize, rinse twice with sterile water, and plate using a P20 with the tip cut slightly to make the opening bigger.
Microtubule dynamics in an Arabidopsis leaf wasteneyslaboratory 528 views Microtubule dynamics in a young leaf from arabidopsis. The plants are stably expressing the microtubule reporter GFP-MBD. Confocal Z-stacks were taken over time and rendere...
Microtubule Dynamics in the mor1-1 mutant wasteneyslaboratory 2,619 views This is a time-lapse movie of microtubules (visualized using a GFP-Tubulin reporter) in the Arabidopsis thaliana mutant mor1-1. Shown is a cotyledon epidermal cell. Colored...
The Microtubule Preprophase Band (PPB) wasteneyslaboratory 2,364 views This cortical ring of microtubules encircling the nucleus forms prior to mitosis. This structure has been the subject of keen interest to plant cell biologists for decades ...
The Plant Interphase Cortical Microtubule Array wasteneyslaboratory 1,969 views Microtubules are nucleated at the cell cortex, and remain attached to the cell cortex along their lengths for the duration of their lifespan. As you can see here, microtubu...
Live Cell Imaging of Cellulase Synthase in a Plant Cell wasteneyslaboratory 2,618 views Cellulose synthesis and microfibril formation are carried out by cellulose-synthase-complexes located at the plasma membrane. One of cellulose synthases, CesA6 is visualize...
Microtubule dynamics in the clasp-1 mutant wasteneyslaboratory 1,628 views Here is a timelapse of microtubule dynamics in a cell of the Arabidopsis clasp-1 mutant, which lacks the CLASP microtubule-associated protein. Note that microtubules freque...
Microtubule Dynamics in Plants wasteneyslaboratory 5,381 views This is a time-lapse movie of microtubules (visualized using a GFP-Tubulin reporter) in a living plant cell. Shown is a cotyledon epidermal cell from an Arabidopsis thalian...

About The Wasteneys Laboratory at University of British Columbia

Our lab is interested in understanding the mechanisms of cell division and expansion in plants. The microtubule cytoskeleton is a key player in governing these events. By influencing the properties of the cellulosic cell walls of plants, microtubules dictate the direction and location of cell expansion. Furthermore, since plant cells are glued together in place and thus cannot migrate, the timing, placement and direction of cell division is paramount in dictating organogenesis and development. Our lab is currently focused on elucidating the mechanisms of how microtubules in plant cells are organized, and how these different organizations affect cell expansion and division.

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