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How NOT to wash a monolayer of adherent cells
be115
1,468 views
Oh, that's not good.
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How to wash a monolayer of adherent cells
be115
2,738 views
Gently, so as not to disturb the cells - just like when we passage cells.
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Saving the cell pellet
be115
1,812 views
It's very easy to accidentally suck up your cells in this step along with your media. Tilt the tube as shown and don't let the pipette get too close to the pellet.
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PBS wash
be115
2,056 views
You want to remove any leftover media because it would interfere with the trypsin in the next step, but you don't want to lose any cells in the process.
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Aspirating media
be115
1,148 views
Getting the media from the flask to the waste bottle.
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Getting a Pasteur pipette
be115
961 views
You can't stick your hand in without compromising the sterility of the rest of the pipettes.
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Hemacytometer loading
be115
7,699 views
Let surface tension do the work for you; don't try to squirt the sample in.
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Hemacytometer assembly
be115
3,345 views
Wet the glass runners to either side with water to hold the coverslip steady.
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Bad pipetting
be115
5,387 views
Take your time!
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Operating the pipette
be115
2,463 views
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