Sigma Life Science
Loading...
79
subscribers
47,299
video views
Featured Playlists
Antibody based Biomarker Discovery in Cancer Research
We recently showed you where to find the wealth of IHC data http://www.sigmabioblogs.com/a<wbr>ntibodies/online-data-treats-h<wbr>uman-protein-atlas/ available from Human Protein Atlas web portal.
Now you can listen to Dr. Frederik Ponten, the Vice-program Director of Human Protein Atlas Project describe an antibody-based proteomics strategy to identifying cancer biomarkers using Human Protein Atlas data.
The strategy combines high-throughput generation of mono-specific antibodies with protein profiling in human tissues and cells using immunohistochemistry (IHC) on cell and tissue microarrays (TMAs). Dr. Ponten covers the development and validation of Prestige Antibodies® powered by Atlas Antibodies and explores the use of these antibodies in the search and characterization of biomarkers related to cancer.
The webinar concludes with a brief overview of Your Favorite Gene Powered by Ingenuity. (http://wherebiobegins.com/yfg<wbr>)
Speaker: Dr. Fredrik Ponten, Professor, Uppsala University and Vice-program Director, Human Protein Atlas Project
6 videos
Play all(6)
6 videos
Play all(6)
Targeted Genome Editing in Eukaryotic Systems
Final chapter of our ZFN webinar series: Targeted Genome Editing in Eukaryotic Systems Using Zinc Finger Nucleases
To register for future webinars, visit http://bit.ly/ZFN_Web1
For more information on CompZr® Zinc Finger Nucleases visit http://bit.ly/compoZr_YouTube
It's a revolution in genomic science. A technique capable of altering the face of basic research and drug discovery. And it's here, ready to help you unlock the darkest mysteries of the scientific universe.
CompoZr® ZFN technology is a breakthrough that enables simple and efficient genomic editing exclusively from Sigma Life Science. Zinc Finger Nuclease (ZFN) technology, allows easy creation of novel cell lines and model organisms with precise and heritable gene additions, deletions or modifications.
CompoZr ZFN technology gives you the power to add, delete or swap your gene of interest. Now it's all possible.
The details:
Rational genome engineering in mammalian cells is of enormous potential across basic research, drug-discovery as well as cell-based assays. Zinc finger nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks (DSBs) at user-specified locations. Within these chimeric proteins the DNA binding specificity of the zinc finger protein determines the site of nuclease action. Such engineered ZFNs are able to recognize and bind to a specified locus and evoke a DSB in the targeted DNA with high efficiency and precision. ZFN-induced double-strand breaks are typically repaired by an imperfect cellular repair mechanism called non-homologous end joining (NHEJ). Due to the low fidelity of NHEJ, a subset of DSBs within a ZFN-treated cellular population will be misrepaired, leading to variable genetic insertions or deletions at the site of the DSB. Drawing from our work with transformed cell lines, primary human cells, and multi-potent stem cells, we will present examples of both single and multiple gene knockouts.
3 videos
Play all(3)
3 videos
Play all(3)
Mathias Uhlen: Towards a Knowledge-based Human Protein Atlas
Sigma Life Science is excited to bring you a recorded webinar of Dr. Mathias Uhlen speaking about the Human Protein Atlas (HPA) program. The HPA is using antibody-based protein profiling based on the human genome sequence data. Through their "high throughput proteomics" they are able to produce protein localizations in human cells, tissues and organs, and many cancer tissues as well.
Thanks to the HPA program, more than 50% of human protein-coded genes have expression data available.
Each antibody produced by HPA contains a summary page for every human gene, a new organ view including 46 tissues and organs and a new cancer view including results from 20 different types of cancer.
The Protein Atlas data is available for anyone to view and analyze at http://www.proteinatlas.org/.
5 videos
Play all(5)
5 videos
Play all(5)
Advertisement
