In a poll that attracted over 84% turnout, Scotland voted on Thursday to remain part of the United Kingdom by around 55% to 45%.
The European Union welcomed Scotland's vote on Friday, ending weeks of silence.
"I welcome the decision of the Scottish people to maintain the unity of the United Kingdom. This outcome is good for the united, open and stronger Europe that the European Commission stands for.” European Commission President Jose Manuel Barroso said.
But fearing that the Scottish referendum might create a domino effect across the continent, Brussels still refuses to comment on other separatist movements, like Catalonia in Spain.
In Madrid, Spanish President Mariano Rajoy expressed his relief at Scotland's decision, as the Spanish government struggles to retain the Catalan push for independence.
"The Scottish have avoided serious economic, social, institutional and political consequences," he said in a video message posted on the government website. "They have chosen the most favourable option for everyone; for themselves, for all of Britain and for the rest of Europe."
Scottish First Minister Alex Salmond accepted defeat early Friday morning, and called on the British government to keep its promise of giving “more powers” to Scotland.
“Just as Scotland will vote separately in the Scottish Parliament on their issues of tax, spending and welfare so too England, as well as Wales and Northern Ireland, should be able to vote on these issues and all this must take place in tandem with, and at the same pace as, the settlement for Scotland.” UK Prime Minister David Cameron added.
Cameron said on Friday that greater powers will be given to the Scottish Parliament, particularly over tax, spending and welfare.