Shotgun sequencing

[howtoshyne]

Does anybody else find it amusing that the big bad private company had to rely on the "public data" to finish? ;)

[liner986]

so how do you complete the sequence the "left out" fragment ends that the computer cant figure out by overlapping?

[christopheradair]

@liner986 primer walking or by filling them in via PCR. This can only be used for small gaps though... more of an educated guess type of deal.

[eucalyptus321123]

hah interesting, i just discussed the repeat problem with a prof of mine. awefully tiring work of completing whole genome sequences

[SpetsnazOXB]

I love genetics

[gtyellow435]

first comment post woohoo