Polymerase chain reaction (PCR)

[MrBenjaminLegend]

where does the nucleotides that the polymerase uses to synthesis the new strand come from?

[chofafernandez]

QUE MASAAAAAAAAAAAAAAAAAAAAAAAAAAA­AA



[hensembulat]

good...

[ManfredSupertyp]

i must say this is very very good! youre a god!



[tz88tz88]

This is very good. Well done!

[H011ycR4p]

This kid needs to get some fucking pussy!

[sweetrevenge191]

@H011ycR4p Are you kidding? it sounds like this guy swims in it!

[sandra02567]

Nice video, Paul! (Matsudaira)...my daughter Carolina liked it a lot...best wishes, sandra

[trinisocababy17]

OMG its now soooo much clearer!!! thanks a bunch i looked at like 4 other vids and this on was the best (y) 

[ViciousOS]

this is a nice, clear video! excellent!

[dancergal9119]

this made it so much more clearer

[watermelancholy]

I GET IT!!!!!!! THANK YOU SO MUCH!

[iluvharraypotta]

omg this saved me, this is so much easier to understand than anyother video

thx thx thx!!!

[fightingnate]

nice mic

[syed810]

I love you

[IvoryInTheSky]

thanksss

[ThinkSodVction]

the best pcr video ever!!!!!!!!!

[BillabongDX]

beautiful! this helped me a lot!

THANK YOU!

[LeeDiddy990]

Thanks this helped me understand the process of PCR without having to kill myself with my textbook. This is going to help with my molecular biology midterm which is in a few hours lol.

[kangmi2311]

it's help me a lot....try pcr song....

[HaleemaMunir]

oh dis iz sooo gud!!

[GeorgeL909]

Thanks for posting this.

[CrushedxIce]

This is awesome. Thank you!

[KARIM0087]

excellent video..must say...thnx pench

[magiccatalyst]

brilliant.

[sidewinder4489]

Thank you so much for this...

You have probably saved me hours of fruitless research and now I think I understand PCR pretty well.

By the way, I found out something while I was researching PCR.

There are other polymerases apart from taq (higher error rate because it can not proofread) that have a proofreading ability and can still withstand the higher temperatures (such as vent and pfu polymerases).

[br4563]

good video it helps to see it visually then reading about it makes it soo much easier.. good job.

[roidroid]

0:52 how are the primers made?

[madcow043]

they are not "made", they were in the mixture from the beginning :)

[roidroid]

What? Of course they were "made"! They didn't just pop into existance all on their own.

Because they seem to be one of the few "designer parts" in the reaction. Everything else seems natural, but the primers will ONLY attach to the specific DNA sequence the experimenter is interested in. So i'm curious how the experimenter makes these primers.

They seem to be one of the few man-made elements in the reaction.

[caseyrainer]

You are right. The primers are design specifically. In one strand of DNA, there are many genes, thus we need to design the primers such that only the target sequence is amplified, not others.

There are many papers discussing how to design the primers. Usually, you have to know the actual sequence before you can design ones.

[roidroid]

Are there specific machines that automate the process? Much like a pcr machine?

Is the required DNA code for the primer input as code manually (like typed in from a keyboard), or is the primer synthesised chemically from a prior existing DNA segment (ie: the required DNA must already exist).

If the latter - how do they target and handle this ONE singular DNA segment? I mean, it's a single, rather short, small DNA segment, it's not like they can look for it & pick it up with tongs right?

[caseyrainer]

specific machine? yes there are, the thermal cycling machine can be bought from Thermo Fisher Scientific.

As for the primers, they are synthesis chemically. Find out how to make a short oligonucleotide from google. They are basically of the same principle.

The ones that I use in lab are purchased from a company which can produce it in commercial quantity. They even give the exact sewuance of the primers in the manual...

[tuddyfruity4]

very good explaination

[carpediem822]

Sweet, thanks!

[mora024]

Ese Kary B. Mullis era bien listo, ¿qué no?

[manyapearl]

wah, thx... im learninG!

[leemurchison]

this is good. nice job.

[barakiji]

esta buenisimo! thnxs for uploading!

[nazioneu]

spettacolo, finalmente ho capito di che cosa mi parla la mia fidanzata la sera...

(translation: wow, finally I have understood what my girlfriend tell me every night...) bye

[anzy256]

this is great! really helped me out :)

[deghty7]

great to see it in a video rather than just reading it

[Imbacrap]

improve my knowledge of Life sci. Thx pal

[pandibear2]

this is great, good job!

[pietrozruski]

mada mada dané

[rollwithbizzle]

thank you

[Monokuroboo06]

visualize... hehe...

[Monokuroboo06]

thanks ! this really helps me to visulaise PCR ...!!

[kamalasumanam]

Thank u very much for uploading