Added: 4 years ago
From: elishanathan
Views: 14,661
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  • @nostanova my phd project was transfecting psm precursors in the primitive streak, I assure you it's possible to transfect specifically. If you reckon it's from a paper, I'll try and sniff it out.

  • What's the red? It looks a little like a lineage tracer, but doesn't seem to move right. Is it transfected?

  • @Confuseddave looks like DI I lipophilic dye

  • @nostanova

    Doesn't look like DiI to me; that would start strong and disappear. The way it comes on slowly makes it look like RFP transfection.

  • @Confuseddave Nah its DII, RFP isnt that red and this video was around before RFP. You couldnt specifically tranfect cells like that in chick its impossible withount tissue transplantation. I remember this video from a paper i read and its DII, I think its by shoenwolf. i worked in chick gastrulation research for 5 years.

  • awesome!! thanx for the vid

  • great video wow amazing

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