Real Time QPCR Data Analysis Tutorial (part 2)

[ufukufi]

what about fold if it is less than 1? for example, by Livak method, we found 2^-ΔΔCt as 0,300. what does it mean?

[americanbiotech]

@ufukufi Typically, it is very difficult to obtain statistically significant differences with fold changes below 1. However, the p-value will set you free!

[americanbiotech]

@waweel, Once you have the data you need to analyze it using standard statistical methodology. Which method you use really depends on a number of factors including how your experiment was run, how many samples were included and how many variables are being analyzed.

[waweel]

After we calculate the fold difference in expression between the control sample and the tested samples, how could we know that the increase or decrease in expression is significant?

[shrawanbhandari]

Great video

[memylan]

Thanks a lot.... great help.

Very simple explanation of difficult concept.

[CarnEHge]

How do you measure efficiency?