Demystifying SDS-Page

[SuperCrazyJunk]

adsasda

[temas61]

efafaw

[aranarthspanish]

"my proteins kind of small"

*snicker*

"Yeah it is"

[assfartis]

dsadsadas

[jenga007beto]

Thanks a lot

[imgoftheinvsbl]

The cake was a lie :(

[mrpapparappa]

U guys ROCK!!! Helped me a lot n keep em comin, please :)!!!

[proteinP53]

muy bueno felicidades

van 5 estrellas

[TOTSDesign]

Great job guys! Funny way to explain the "misterious world" of dear SDS-Page...See ya!

[johnnieguy09]

Nice video, but I can't believe you are A) Wearing shorts in a laboratory and B) eating cake in a laboratory. Didn't you learn anything in safety training? lol

[aesopfables4]

woot woot cheers for slb :]

[rsobhee]

Thnx for posting a dumb loud music in the background.. it made it easier to hear what you were talking..

[ahnatanha]

do i love you guyz or what!! ;)

[Arammik]

The "one last concept" is actually the main concept of the laemmli system. Instead of running around in the forest, you guys could have explained that for about 5 mins in the video. :)

[Polarcupcheck]

I like, and dislike, the explanation of the stacking. I get it in the context of this video, but I'm still worried that isn't the whole story, and may even be false. I never took physical chemistry, so maybe that is where the explanation is.

[Polarcupcheck]

You guys seem to explain it as a kind of electron shielding.

[ianm12]

Nice work!

[dwang008]

cool video, i learned alot. sorry for what may like "noob" questions but the chloride ions and glycine buffers, where do they come from? are they part of the protein solution or the gel? also, what is the significance in knowing the molecular weight of protein? How do researchers utilize that information?

Im taking an intro biochem class right now and we havent covered this but im pretty interested.

[michaelatm2603]

I'm taking molecular biology class right now. I'm new to this,too. I think we can sort that protein based on its molecular weight and its pH, so we can further analyze what does that protein do based on protein library. For the buffer, I think it's a part of SDS Page itself. You can see they add it at the beginning.

[azncount]

Hey guys AWESOME video!!! Thanks so much for clearing up the confusion I had :) and yes that cake looks really yummy.

[capkeez]

I want some of that cake. And awesome video. Cleared up some stuff for me. Thanks!

[disinterested22]

he's a giant!

[justintime303]

judy is mad cute haha

[eluminusunknown]

confusing video.............priceless

[DYANNN8]

I don't think it was confusing at all. It was very helpful. Thanks.

[berlin45ers]

my prtein is kind a small hahahahhahaha

[Fatmob79]

UC DAVIS...sweet. I used to live in Arlington Farms near the "gel" site. :P

[eliash1988]

best explanatory video, helped me in lab report.....keep it up

[samipisces]

Really very helpful for beginners. Thanks for uploading such a wonderful video.

[BusinessTime49]

Very helpful!!!!

[mosamy85]

nice one guys ... quite funny aswell

[1965maple]

Most inadequate (and incorrect) explanation of how stacking works. The idea is that conductivity of stacking gel has to be much lower than that of resolving - meaning voltage drop (electric field intensity and thus rate of electrophoresis) is higher in stacker. Hence the concentrating effect - proteins in stacker move fast while those that reaches resolving "stop" in comparison. This is done by pH/glycine in stacker. Read up on Ornstein-Davis system (Laemmli's only modification was to add SDS).

[gonegoing123]

Thanks guys...

[harm82]

well Sodium Dodecylsulfate has a charge of -1?

the polymer gel is represented by white separated balls? the dodecylsulfate has a spherical shape?

are you apple users (physicists)?

[rax2099]

lol

i know what you mean...

they're just trying to explain to people who doesn't know anything about electrophoresis.

[ssssssss920]

thankksssss ı m so glad to have thıs video ı have an exam from this topic thankksssss :D:D:DDDDDDDD

[canomunu]

thanks for this video ı have an exam from this(sds-page)

[hartiganutube]

You are amazing!Great job!

[legatoJR]

Really helpful guys, thanks so much!

[Xirj]

Ahaha, I now finally understand why there's stacking gel :).

[n0r3k]

Masterpiece!

After this film everything is easy and clear.

[dejaflu]

Pretty good SDS Page lesson, except for that last part...when you ate cake in the LAB. Tisk tisk.

[sangbums2]

whats this song called??

[bs729]

this was fun!

[ChristineWrites]

I LOVE this video. Thanks Guys!

[befz88]

i have a small stick too but i never win

[saketjoy]

which lab it is man...adjoining a nice bushy forest!!

[dizue]

nice analogy of SDS

[kitajimasaburo]

hey!! is it wise to eat in the lab? :)) nice and teaching video. thank you so much

[aruaco2000]

Hi, excellent analogy.. thanks ! ...ha y tu Judy eres increiblemente hermosa ;)