Key Steps of Molecular Cloning

[shoppittsburghnow]

great vid you have here

[staranjela]

really informative and interesting

[alexasmithy]

very interesting video thanks

[ntlema2]

couple of questions:

How do you determine which restriction enzymes to use?

What liquid medium was used in the end to extract the cloned DNA?

[CloningStrategies]

@ntlema2 Great questions!

Which enzymes to use: This is what you decide as a scientist when you are planning your cloning experiment. You look at the sequence of the plasmid and see what restrictions sites are available, then you integrate those sites into the primers you use to amplify the DNA fragment. Check "Simply Cloning - Chapter 1 - Planning" video.

To extract cloned DNA: It is more than just a liquid medium. Check "Chapter 8 - Plasmid Miniprep"

[eckostar61]

is there any video on expession DNA cloning?? please need help with it

[pawangurway]

simple... usefull... n effective !!! aWESOME !!!!

[powerzoner]

@CloningStrategies why are two different REs used?

[CloningStrategies]

@powerzoner If you stop the video at 2:58 min you will see that overhangs from different restriction enzymes don't match each other. So plasmid vector can't close on its own, only with the insert.

There are all kinds of exceptions, but using two REs is a simple way of preventing vector from closing onto itself.

[powerzoner]

@CloningStrategies Oh I see! Thanks man, great vid.

[akhyaar12]

Thank You Buddy. U made Molecular Cloning Interesting

[tessawyllie]

@CloningStrategies Do one on cDNA and gDNA libraries!

[SabzoM]

THANK YOU SO MUCH!!! i have to do an essay on this, and you really helped me..even more than my own lecture notes haha :)

[bhimgd]

helpful... good.. thank you very much

[bhimgd]

helpful... good.. thank you very much

[spyfox0001]

Very good video quality and detail, although some parts are longer than they need to be because they wanted to show cool graphics.

[Wowhimpowwow]

lame. i like the pacmans though :)

[youtubyfein]

this video was soooooo helpful !!!

[Ponezorg]

Thank you for being awesome

[tudoralexe]

as everyone above said: very good video. very clear information.

[Faby07091981]

ottimo video!!!!! 

[Reed12223]

Great video! Really straight forward :)

[johnmc2k]

Great refresher video!

[kitCD]

loved it. Thankx alot. it was very helpful

[billowingsatire12]

Incredible tutorial!

[feb17aaru]

This is such a helpful video!THANK YOU SO MUCH!

[CloningStrategies]

@feb17aaru

- Hey, you made my day :-)

[elmariachirockerocho]

Very well-made.

[mcca1674]

Thank you! I wished I had watched this before my interview!

[proteinP53]

very good video!!!

[ChommyK]

Very helpful video.

[foeasy16]

soooo DNA Ligase is pacman? Finally makes sense now

[nalinirau]

would they not have cloned if the insert had not been added in?

[vanopnt]

i like it avery much. very descriptive.

[EmbryoJenya]

До боли знакомый голос :))

Seriously: Every bio, biochem etc. freshman MUST see this video!

[biochemisery]

I really like this animation because it makes everything simple. I was rather confused before that.