how a zebrafish embryo is dechorionated

[CAIntegrity]

When I dechorionate my zebrafish embryos, I just use two pairs of little tweezers. They work much better.

[DrSoez]

YOU KILLED IT!

[icecub3]

@DrSoez all in the name of science.....

(not that i signed up for it btw)

[icecub3]

@DrSoez all in the name of science.....

(not that i signed up for it btw)

[bigdude9876]

My professor just told me to watch this to see how its done...for later when i do it. I give you props and thanks for the info.

[icecub3]

@bigdude9876 no problem! ur comment just made my day! =D

[leTroiseme]

looks like its about 30-35 hours old? I just did the same thing today. Amazing!

[rosydonut]

Woah..Now THAT is delicate work..And I thought sewing was fiddley!

Lol...

[chaudharyavdesh]

Hi is Protease and Pronase same? Is there any one who can help me answering this question.

[redvtec03]

Pronase is an enzyme that degrades the chorion. You have to be careful though to rinse the naked embryos thoroughly afterwards to avoid degredation of their actual bodies. Works GREAT as long as you know what you are doing!

[cmart989]

Agree with the last comment. I use little tweezers to do this. It's better than using what you have. But I also have destroyed lots too....

[frogfactory]

It'd be way easier to use two pairs of number 6 watchmakers' forceps than those stick things. It must take you forever to get through a batch of 200 doing it that way. With forceps you can dechorionate in a second or two, once you get into the flow of it.

[icecub3]

haha well lucky for me the dechorionation isn't the main focus of the project so we only needed to dechorionate a few. if i rmb right my project was to expose them to some compounds of an anti-SARS drug to see if there were adverse reactions to the zebrafish..

thanks for the tip anw!

cheers,

chris

[MultiMomo1990]

could you please help me . at my school I have to do that for my group project and I dont know how. could you help please

[madwithpower]

nice one cubie. haha :D

[icecub3]

hey haha thx mad! =D

[mwest1234]

Can you tell me what kind of microscope you used and what kind of forceps?

[RNakkula]

Thanks for the video! I used to work in a lab that studied these little critters, but when the funding ran out, I got a different job.

[icecub3]

thats cool~ haha sure no prob! xD

[LaReine3335]

I know exactly what you're talking about. It's much harder than it looks. It's so much easier to use pronase.

[icecub3]

haha sorry what's pronase? how's it work?

[MFSilverOxide]

You gotta do what you gotta do for science...

[cr4sh0v3rr1d3]

Poor little guy!

[Gatman1]

What species of animal did you get this embryo from?

[icecub3]

oh sorry dint mention it. it's a zebrafish embyro =)