Gel Electrophoresis

[Balkance]

great music! :D

[defranco23]

what a terrible music.....

[marcbinsen]

@defranco23 "what a terrible music" Great sentence dickhead

[Yesaul19]

Hell yea! Another molecular biologist that likes Gogol Bordello!!!

[Excalibur317]

how come some DNA moved to the positive end of the electrophoresis chamber? :o

[nnutcase]

@Excalibur317 what do you mean? It started out with two rows.

[SoundsLikeSubstance]

@Excalibur317 DNA has a negative charge so it moves towards the positive end of the gel. Restriction enzymes cut the DNA at specific restriction sites. The smaller the fragment of DNA cut by the enzyme, the further it can move towards the positive end of the gel, kind of like a filtration device. :)

[irnigemzon]

@Excalibur317 DNA has a constant negative charge on it so it tends to move towards the positive (cathode) side of the gel

[jalsasravan]

@Excalibur317 dna is -ve charged buddy

[VolkgartenBySquirrel]

I could do with some more of this record - thanks for your demo!

[karnavinash]

didnt see any bands except the ladder :(

[lilimercedes]

to be honest, the music just distract from the lesson!

[xtaxinyoface]

You touched the gel with the glove which you pressed the buttons with... ?/ Thats not good, i think. Theres Etidium Bromide in the gel which bonds very fast and good wiv you DNA. Reaction please ??

[nnutcase]

the lab has a rule of always wearing gloves whenever working. Even when touching buttons, exactly for that reason.

[xtaxinyoface]

Ohh okay but then i dont know the english word for it but its filthy then so whenever someone presses the button wiv his hand its not good then .. You see what i mean i study this in the netherlands maybe the rules are bit more strict here .. :S Good vid though ;)

[imass08]

@nnutcase I think he meant you should change another glove. Of cos u have to wear gloves while working.

[nnutcase]

@imass08 I'm assuming the next people are wearing gloves, anyway :). Naw, it wasn't the cleanest lab in the world. You guys are right.

[tomprman]

helu what is the name of the crystal bottles with red cap? to buy it?

[YZerker]

lmao

[chanace]

What is this song?

[nnutcase]

Super Taranta by Gogol Bordello

[chanace]

Cool. Gogol sounds Russian

[nnutcase]

Some of them are. Nicolai Gogol was a Ukrainian who wrote literature in Russian, but injected it with a lot of Ukrainian culture at a time when it was unacceptable. The band name strategically used Gogol's name because of how multicultural their music is. They are a collection of musicians from around the world. Lead singer is a Ukrainian guy with gypsy roots whose first language is Russian, who has moved from Ukraine to Italy to NYC.

[calam05]

toxic, high voltage, jumper tied around waist. this is extreme electrophoresis

[smerrigan]

wouldve helped to explain what you were doing instead of setting this to music..i learned zero

[nnutcase]

how about you read about it, and then rewatch it and try to figure out what's going on. I'm not here to teach something to you that you can learn in a more active way yourself.

[itsumonihon]

nah, trust the viewers, it's better if you get rid of the music and narrate it

[nnutcase]

@itsumonihon it wasn't made to teach, but to demonstrate

[ddjsrob]

yeah

[Lapsigone]

how ridiculously badass ;)

[dandaman989]

Very fun.

[stepb24]

I love working in a lab! :)

[Xendillian]

Thanks a lot for this! It's very hepful!

And it must have been really fun doing it!

[hk8900]

I used to pour liquid agarose gel solution into the square container before placing the combs to set the wells ;)

[jplatter14]

how much does a micropipetter cost?isn't like 200-400?

[das2786]

i already exposed to that toxic! EtBr thing dude....that's suck!!!!

[Bowserdude1980]

that shit is fun

[HungryLama]

uhm.. one question. Did you add the running buffer to the gel before you ran the DNA?

[nnutcase]

It's 1% agar with running buffer to make the gel, yes. We stain it after it hardens to avoid ethidium bromide being in more than one place, but I know that other labs add the ethidium bromide directly to the gel to make the fragments look neater.

[HungryLama]

oh i see. so the Toxic! thing is the running buffer but do you add it again after the gel hardens? Like i read somewhere that you have to submerge the hardened gel in the buffer while it is in the cradle...

[nnutcase]

No no no, the toxic thing is ethidium bromide. What they meant was that to actually run a gel, you submerge it into the running buffer in the cradle, pipet the samples into the gel while under the running buffer, then close it up and start it, and the electrical current runs through the running buffer AND the gel. That's where you saw the bubbles at 2:20 -- the gel is under the running buffer.

[HungryLama]

Oh I see. Thanks for the info! :)

[usernamousss]

do any of u guys know wat the song is called?

[nnutcase]

It's "Super Taranta" by Gogol Bordello

[Scatterhearts]

this was very helpful!

[lawtonette]

ooo - the video and biol stuff are cool, the musics almost as cool! who is it?

[nnutcase]

It's Gogol Bordello

[jhirkus]

asds

[benjaher]

Nice Molecular Weight marker!! because there are not DNA!!!its a lot of agarose for only to watch the molecular marker!!!

[nnutcase]

I know, right? I only had one shot to make the video, so it not coming out was a minor "detail." Eventually I got some fragments, but it was too late.

[pamont2]

Nice vid, I started this lab in my bio class yesterday. Pretty interesting, and now i have a head start =)