Cutting with a microtome

[mesholmesho]

I always using the needle to spread the cutting tissue , but I will change to the brush because I always cutting the tissue with the needle

Thanks

[brandonjjun]

Awesome! I'm having trouble sectioning brain tissue in paraffin. I always end up with wrinkles or some kind of puckering when I mount on slides and also the sections roll over on themselves while I'm slicing. I read you suggesting refrigerating the blocks beforehand? help please!

[AtheneWinsFan]

Fantastic!

[FatalMissEllen]

Wow thats so much better then I can do in school ^_^

[RONALDSCHULTE]

@FatalMissEllen

Thanks, All I can say about that is practise and practise. My first coupes where really bad but after a lot of practise it became better and better. Important is to cool the block in the fridge.

[donehardy]

leica blades haha

[Motorcycliste]

This the machine my butcher uses at his deli. The cheap bastard cuts the pastrami this thin...

[TheLAPosh]

@Motorcycliste ahahahha!

[ibnsinalab]

we have thick lymph node sections. How can we improve our sections?

[rezaeijavan]

This is a USEFUL video on youtube

[Princisje]

Leuk dat je microtoom video's geplaatst hebt. Ik moet deze week aan de bak met coupes snijden maar ben nog een leek. De filmpjes zijn heel behulpzaam. :-)

[RONALDSCHULTE]

@Princisje Dank voor je reactie, wat doe je precies? Ben benieuwd naar jou techniek en ervaringen. Op mijn website (ronaldschulte.nl) staat nog veel meer histologie en technieken. Daar kun je me ook via mail bereiken. Groet Ronald

[Princisje]

@RONALDSCHULTE Ik heb nog helemaal geen ervaring met snijden. 1 keer geoefend. Er moet nu veel gesneden worden dus iedereen wordt een dag ingezet net zo lang tot alles gedaan is. 

[crzyp852]

is it cheese

[612jo]

this microtome is so retro ;) check out Hydrax M40 (Zeiss), my favourite out of those 4 I work on

[mujjuman]

Cool, but aren't you supposed to cut more slowly?

Also, my tissues seem to be shredding apart when I cut. How can I fix that? Is there a website I can go to to get some tips on cutting, mounting, and staining?

[UkyoKuonji2010]

Nice clean ribbon! Why can't my microtome make something like that? I end up wearing most of my ribbons or it ends up all over everything(including the microtome) XD Cooling the block doesn't help nor does changing the blade. I guess it's the kind of paraffin wax we use or something.

[RONALDSCHULTE]

Hello,

I use paraplast but use of normal paraffin is fine to. Of course is my cutting not always a party. Speed, umidity, temperature, thickness etcetera. Al those things must be perfect. When the air is to dry you can't cut one good coupe. Lung tissue gives better results than a hard Sternum. Start with 7 or 8um. That should always give a good coupe. If not than is your knife angle maybe to great or small. My angle is 32 degrees. Gr Ronald

[UkyoKuonji2010]

We have been just using normal paraffin so far. I try to keep my ribbons 5 micrometers thick and I try to average one slice per second. Lung tissue is pretty easy, and I found kidney tissue wasn't that bad either(but that could just be me or something). I make sure my clearance angle is around 2.5, it doesn't go higher than ten or something. I know it's not the actual degrees. We do everything in room temperature but we keep our blocks on ice when we're not using them.

[AussiePersian]

Not sure if you were cutting slowly for demonstration purposes but I would recommend cutting faster to get a better ribbon.

[RONALDSCHULTE]

Not only for this video. Sometimes tissue cut better with a slower speed, sometimes not.

[jerryleelewisfan42]

thank you !

[gizzel345]

Is that a tumor?

[RONALDSCHULTE]

This is a part of a smallbrain (cerebellum)from a cow. Ronald

[LittleMissAngry]

Great video :) I didn't think someone would post what I do every day on youtube, but you did!

I kept wincing every time your hand went near that blade though. I just *chunked* my pinky finger. Ouch.

[nohamakhlouf]

I liked the video so much, the tissue sections look great and the ribbon is terrific.