@baselta1 About 48 hours. MM14 cultures will spontaneously differentiate upon serum withdraw (1.5% horse serum). This particular culture was actually "self-conditioned"; meaning that the culture was allowed to proliferate under growth conditions (85% Ham's F10C, gentamycin, 15% horse serum and 2ng/mL bFGF) and then allowed to self-deplete local growth factors by simply not subjecting the culture to repeated medium changes.
How long is this time lapse movie? In another word, how long did it take the cells to change from a single myoblasts into myotubes?
What was the differentiation (fusion) media?
Thank you
baselta1 3 months ago
@baselta1 About 48 hours. MM14 cultures will spontaneously differentiate upon serum withdraw (1.5% horse serum). This particular culture was actually "self-conditioned"; meaning that the culture was allowed to proliferate under growth conditions (85% Ham's F10C, gentamycin, 15% horse serum and 2ng/mL bFGF) and then allowed to self-deplete local growth factors by simply not subjecting the culture to repeated medium changes.
ptai 3 months ago