I have seen this done manually. They use a larger vial, at least 10 ml and they use a septum top and drive the syringe through the septum.. The larger vial takes longer to heat up, but still not very long.
They make autosamplers for this, the usual approach.
Quantitation under headspace is always done with an internal standard.
Notice that no care is taken to thermostat syringe or sample. While equilibrium headspace sampling is a useful technique, anything that disturbs thermal equilibrium invalidates the sample preparation.
@starchingllc I shouldn't have said Air. I should have said the He. My bad. The injection port is pressurized and that could have caused the pumper off. I have seen it before. I dont know why you mentioned the FID though. The Detector is no where near the injection port
@lilpolo20 Yes, he should not leave the syringe on while press start button. but injector temp is usually 50 degree higher than the BP, whatever injected is gasified immediately, and it won't blow off the septum. I do not know what is pumper, i guess you mean syringe or septum.
@starchingllc The pumper on the syringe. The part that pulls the sample in the syringe. The pumper on the syringe can be blown off if it isn't done quickly. And if the pumper gets blown off before the injection, then there is no sample to vaporize in the vaporization chamber. I have seen it done plenty of times my friend.
@starchingllc watch the video again. As he is injecting the syringe, he is not using his pointer finger to make sure the pumper thingy on the syringe doesn't shoot out because of the pressure in the injector port. That is the part that im talking about. And after he has made the injection, his finger is still off of the pumper. I work with this instrument every day. We have an injection tower but I have done hundreds of hand injections before.
I have seen this done manually. They use a larger vial, at least 10 ml and they use a septum top and drive the syringe through the septum.. The larger vial takes longer to heat up, but still not very long.
They make autosamplers for this, the usual approach.
Quantitation under headspace is always done with an internal standard.
mrphysh 8 months ago
@fannykok it's not the same... u dont inject gas in a Liquid Chromatograph..
Hornypuff 1 year ago
Notice that no care is taken to thermostat syringe or sample. While equilibrium headspace sampling is a useful technique, anything that disturbs thermal equilibrium invalidates the sample preparation.
HCCCHEM01 1 year ago
this guy was messing up. air could have shot throw the syring and blew the pumper off. I have seen it happen too many times.
lilpolo20 2 years ago
@lilpolo20 what are you talking about. you need air for FID anyway. That tiny amount of air from the syring is nothing.
starchingllc 5 months ago
@starchingllc I shouldn't have said Air. I should have said the He. My bad. The injection port is pressurized and that could have caused the pumper off. I have seen it before. I dont know why you mentioned the FID though. The Detector is no where near the injection port
lilpolo20 5 months ago
@lilpolo20 Yes, he should not leave the syringe on while press start button. but injector temp is usually 50 degree higher than the BP, whatever injected is gasified immediately, and it won't blow off the septum. I do not know what is pumper, i guess you mean syringe or septum.
starchingllc 5 months ago
@starchingllc The pumper on the syringe. The part that pulls the sample in the syringe. The pumper on the syringe can be blown off if it isn't done quickly. And if the pumper gets blown off before the injection, then there is no sample to vaporize in the vaporization chamber. I have seen it done plenty of times my friend.
lilpolo20 5 months ago
@lilpolo20 thx for replying your post 2 years old. I never see this happen, but its good to know.
starchingllc 5 months ago
@starchingllc watch the video again. As he is injecting the syringe, he is not using his pointer finger to make sure the pumper thingy on the syringe doesn't shoot out because of the pressure in the injector port. That is the part that im talking about. And after he has made the injection, his finger is still off of the pumper. I work with this instrument every day. We have an injection tower but I have done hundreds of hand injections before.
lilpolo20 5 months ago
lol cool!!!! i took that in science today lol!! and i am in grade 7!! its so awsome
sakonpure6 3 years ago
is it same way with HPLC?
fannykok 3 years ago
please add details . The syringe filling part is not very clear. Please show us the technique.Thanks for the video.
chandnee 4 years ago