@LordStephanos What's imaged in these and similar experiments are beads attached to the molecule of interest. The bead here, while small, is larger than the wavelength of light and so is visualizable with a light microsope. The F1 ATPase spins the bead that is attached to it, which is what the movie is showing. By analyzing the bead's motion and its hydrodynamic drag through the surrounding fluid, one can even figure out the torque the ATPase molecule is applying.
Light microscope? Molecules can't be seen on light microscope.
LordStephanos 9 months ago
@LordStephanos I'm guessing high-speed atomic force microscopy.
MrRobotoToo 9 months ago
@LordStephanos What's imaged in these and similar experiments are beads attached to the molecule of interest. The bead here, while small, is larger than the wavelength of light and so is visualizable with a light microsope. The F1 ATPase spins the bead that is attached to it, which is what the movie is showing. By analyzing the bead's motion and its hydrodynamic drag through the surrounding fluid, one can even figure out the torque the ATPase molecule is applying.
rp099 9 months ago
Awesome!
Elusefelier 1 year ago