lol we're doing this for our showcase project at cart. it's not that its hard, but you just gotta be careful on what your adding. for us, we have to start from scratch. making the blotting buffer, sample buffer, primary antibodies, secondary antibodies....yeesh. this is such a long project and a bit hard to understand.
@salesmilton Based on my understanding of Western blot, you would only want to do one sample at a time b/c you're detecting an antigen. If you had samples from 5 people, say, and it turned up positive, how do you know which of the 5 has the antigen? Maybe all 5 do, maybe only 1. You'd have to test their samples individually to know.
@salesmilton Actually, I'd like to amend my previous statement--I do think you can run multiple columns on the gel at once, each column with a different sample, potentially samples from different individuals.
Karyotyping sounds amazing... though it is probably contamination prone considering the process. That's not something they even mentioned you could even do at my university. Thanks for sharing that bit of into.
We learn those techniques (Microarray, Western Blot, Gel-electrophoresis, Southern and Northern Blot) in university theoretical and later practical and it's interesting to see it how it works.
@XSirApocalypseX - that's how it works at my uni, too! We only briefly discussed Western blotting in a grad level course I took and now I'm actually doing Westerns in a lab! It is definitely much more different than what I imagined.
lol we're doing this for our showcase project at cart. it's not that its hard, but you just gotta be careful on what your adding. for us, we have to start from scratch. making the blotting buffer, sample buffer, primary antibodies, secondary antibodies....yeesh. this is such a long project and a bit hard to understand.
mouaynicholas 1 week ago
well... ok
ZMVictoria298 2 months ago
nice vid
fPMaryanne393 2 months ago
actually at 2:48
gnarkillw 3 months ago
is sodium in the SDS being repelled by the positive electric current? and that is those bubbles at 1:54?
gnarkillw 3 months ago
Thank you so very much, whoever posted this. It really helps a lot.
moearly 4 months ago
perty music
maraud48 5 months ago
non c'ho capito un cazzo!
andpie89 5 months ago
Preciso, educativo, genial!
muchas gracias!
intrascendenthal 8 months ago
this song goes hard... lol jk. this video actually taught me something.
yorellana93 8 months ago
very complicated.
ivanwolff 9 months ago
thanks alot, got a lab on monday on doing this, thanks very much. . . . more than prepared now :), i will own
luffinbitbybit 9 months ago
too complicated
prudvipeddireddi 10 months ago
Is this test used for a single patient or multiple patients? If multiple, what is the maximum number of patients that can be tested.
salesmilton 11 months ago
@salesmilton Based on my understanding of Western blot, you would only want to do one sample at a time b/c you're detecting an antigen. If you had samples from 5 people, say, and it turned up positive, how do you know which of the 5 has the antigen? Maybe all 5 do, maybe only 1. You'd have to test their samples individually to know.
moearly 4 months ago
@salesmilton Actually, I'd like to amend my previous statement--I do think you can run multiple columns on the gel at once, each column with a different sample, potentially samples from different individuals.
moearly 4 months ago
Seems easy in the video. Way different in a lab like mine.
danielhdoro 1 year ago
I miei trasferimenti escono meglio anche senza tutte queste bellissime makkine e accessori!
crilly87 1 year ago
i have a test about this tomorrow i did it in the lab but this was waaay more comprehensive thanx!!!
reinaldofranqui1 1 year ago
1:27 her sleeve's got stained :P
JNDION 1 year ago 2
yes very superb professional handling skills. i'm gonna go into lab work.
samsaras 1 year ago
wow what a demosntration superb its purely profesonal handling
ravitej09 1 year ago
Karyotyping sounds amazing... though it is probably contamination prone considering the process. That's not something they even mentioned you could even do at my university. Thanks for sharing that bit of into.
nicklinkzelda 1 year ago
We learn those techniques (Microarray, Western Blot, Gel-electrophoresis, Southern and Northern Blot) in university theoretical and later practical and it's interesting to see it how it works.
XSirApocalypseX 1 year ago
@XSirApocalypseX - that's how it works at my uni, too! We only briefly discussed Western blotting in a grad level course I took and now I'm actually doing Westerns in a lab! It is definitely much more different than what I imagined.
nicklinkzelda 1 year ago
@nicklinkzelda That's really interesting, but what I want to do is to do a karyogramm, we've genetics, als human-genetics. Sorry for my bad english.
XSirApocalypseX 1 year ago