you should be carefull when adding microwaved water to another medium as it can be superheated with no pitts to change state in the smooth glass flask
Veri nice video. I see before autoclaving, the girl is closing the tissue culture jars and the caps are not placed completely, i mean some sides are still up. is it done intentionally? i want to use baby food jars. should i place and close them tightly or should i just put the cap withought tighten it before autoclaving? Thanks phytotechlabs.
@Gemthermakleingk Yes, this is done intentionally with polypropylene containers like these. The lids seal tightly which results in deformed containers when the pressure drops at the end of the autoclave cycle. This is circumvented by leaving the lids slightly ajar and then closing them when the autoclave is opened at the end of the cycle. This is not a problem with baby food jars using our PhytaCaps or Magenta B-Caps.
Most plant growth regulators are routinely autoclaved in the medium, especially the stable ones such as BA & NAA. Some are heat labile and should be filter sterilized and added after the medium is autoclaved and cooled a while. Our "Plant Growth Regulators, Part II" document on the Technical Info page on our web site offers guidelines for whether the plant growth regulator can be autoclaved in the medium or filter sterilized.
you should be carefull when adding microwaved water to another medium as it can be superheated with no pitts to change state in the smooth glass flask
Rannyfash 2 months ago
Excellent presentation.... ..i suggest you to prepare more video clips like this to educate the people those who work with this field...
Thank You Very Much.
Bimal....
athulabar 5 months ago
Veri nice video. I see before autoclaving, the girl is closing the tissue culture jars and the caps are not placed completely, i mean some sides are still up. is it done intentionally? i want to use baby food jars. should i place and close them tightly or should i just put the cap withought tighten it before autoclaving? Thanks phytotechlabs.
Gemthermakleingk 1 year ago
@Gemthermakleingk Yes, this is done intentionally with polypropylene containers like these. The lids seal tightly which results in deformed containers when the pressure drops at the end of the autoclave cycle. This is circumvented by leaving the lids slightly ajar and then closing them when the autoclave is opened at the end of the cycle. This is not a problem with baby food jars using our PhytaCaps or Magenta B-Caps.
PhytoTechLabs 11 months ago
autoclaving the media including plant growth regulators causes hormon deformation.
340070007 1 year ago
@340070007
Most plant growth regulators are routinely autoclaved in the medium, especially the stable ones such as BA & NAA. Some are heat labile and should be filter sterilized and added after the medium is autoclaved and cooled a while. Our "Plant Growth Regulators, Part II" document on the Technical Info page on our web site offers guidelines for whether the plant growth regulator can be autoclaved in the medium or filter sterilized.
Gary Seckinger
Technical Services
PhytoTechnology Labs
PhytoTechLabs 1 year ago
Thank You so much for Your continued effort with these videos!
Please keep them coming.
Gary
Garden City, KS
goldslinger 1 year ago