High Performance Liquid Chromatography HPLC

[IamFreeRu]

What is the price of a unit like this?

[wwwRSCorg]

Please email library@rsc.org and they will help you with your enquiry

[tgkillings]

British people are good at explaining things.

[nelrish24]

wonderful! 

[282sanjay]

is their any method for the analysis of thyroid hormone like T3, T4, TSH and calcitonin using HPLC Technic. if yes then please help me by giving the prepareation and appropiate column for the analysis of the above from serum sample

[seadonkey1987]

@282sanjay

Yes you can use HPLC to measure these. However, I work in a hospital and we measure T3, T4 TSH and calcitonin via immunoassay. Its a much cheaper, faster and more sensitive method. You can obtain results within 30minutes, compared to HPLC which takes atleast a few hours and more working time to extract

[zagaroooo]

bravo bravo score

[NashvilleKildCountry]

I'm amazed at what software has done for these things over the years. Anymore you almost never need to touch the controls on the components. This new controller we got in our lab just lets us set up our methods, standards, unknowns, and whatever else and then runs the batch with more samples than you would ever care to run. I can even have it run a shut down method to flush the column, cool the oven, shut the lamp off, etc. It'll do everything short of having your coffee ready in the morning

[08robelle]

What model of HPLC is this? thank you.

[hplcflowmeter]

thanks again

[hplcflowmeter]

wonderful

[MrDirkles]

Brilliant video. Very clear.

[muhammad922]

amazing

[deathempire70]

Can HPLC be used to extract the individual ingredients from the injected solutions?

[Yibitech]

@deathempire70 You can use HPLC for isolation though its not very efficient for large quantities. Because materials in the mixture go through the stationary phase at different rates, they separate.  As one chemical begins to come off of the column, the detector begins to draw your peak, as long as you have good baseline separation you can collect that in a flask. You collect your mobile phase and desired chemical and use a rotary evaporator to remove mobile phase from the desired chemical.

[saurabhCherished]

@Yibitech

So which technology is used for large quantities?

[jnielsen20]

@saurabhCherished I would suggest normal chromatographic columns, if the product is well known, an affinity column might be aqble to do the job.

[lamelameful]

I need to determine the level of antioxidant (a-tocopherol) in an unknown substance. Is this the most effective way to calculate the level of a-tocopherol or are there some other ways?

*I need to know the level of antioxidant and NOT the presence of it. Thanks :D

[Yibitech]

@lamelameful Yes HPLC can be used for quantitative analysis. You can calculate using the method of internal standard or external standard, external standard being the method mostly used with HPLC. However for a-tocopherol, which I'm not very familiar with, gas chromatography may be more suitable.

[lamelameful]

I need to determine the level of antioxidant (a-tocopherol) in an unknown substance. Is this the most effective way to calculate the level of a-tocopherol or are there some other ways?

*I need to know the level of antioxidant and NOT the presence of it. Thanks :D

[kapaya100able]

great video!!! please put more. Thanks.

[sxcosssxs]

thank you very much.

[crazy25000]

thanks for the video!!

[sateeshmsc]

great



[shabutie1337]

My HPLC consists of a copper tube column, cigarette lighter for heating, spit as the mobile phase, rubber cement for the stationary phase, and my coworker blowing through the end as motive force; our detector is is a white piece of paper...so far, it is not so accurate, but we are going to make an IPO as soon as it works.

[danyeeful]

GREAT!!!!!!!!!!!!

[twtmei88]

Thanks! this really helped. I wonder how they use this along with MS.

[Lima547]

Thanks for posting! Very Informative!

[Lima547]

Very very good! Thanks....

[mongaakshay]

superb...could not get the topic earlier but after seeing the video its just lik doing the topic for 100th time...well explained...

a chart at the end just for a brief or overview of all process would also be great...

[alokrajwins]

its really great

[kittithapa]

thank you. it was helpful

[9665851477]

awesome

[craneywatch]

The presentation was great, and the lab work too. But there were some glitches. The chart didn't show perfectly defined peaks for the strong substance. Then, the needle of autosampler was not shielded.

[ShaSowmya]

brillant work,very informative and thanks

[azhamuthu81]

great work.....

[oo00SAMB00oo]

thnx helped a lot with my chem cswrk!

[MrJason1234]

Great video, simple explanation. Was hoping for more of an explanation for column selection, mobile phase composition, and other factors influencing compound separation.

Would love to see a video for spectrofluorimetry.

[twistertheman]

Very amazing video nice and simple , but why u didn't add the applications of the HPLC it would be of great important to those who use it in pharmaceutical industry ,,

[ahmedkhames]

very very useful vedio

chemist: ahmed khamis EGYPT

[zhanghi]

Excellent, very informative

[781967kh1]

great for university students in the first and second grades

[sweetprinci]

brilliant.

[kenvialsaybar]

great help for lab act tom. thanks a lot!

[manupalace]

lifesaver

[Blahnala]

this is a great explanation of the process, and I'd like to paraphrase the experiment performed in a paper I'm writing(and give due credit), may I do so without buying the dvd?

[sas911000]

thanx for the video

[BostonMA1979]

Great video. Very simple explanation.