Topoisomerases

[AZNKC]

pharmacology

[mazuiface]

BIOL 4003 here

[zenithar6666]

what would happen if topo was not there? would supercoilding prevent replication  base pairs would no longer be exposed?

[mari064]

Oh see, I can play snake during it loads !

[mobcat40]

holy shat that makes sense

[tmac2k61]

Thumbs up if BIO 308 brought you here...and thumbs up for a FUCK YOU towards the upcoming exam also.

[ajo153]

@tmac2k61 Steiners Bio 315 lol

[zenithar6666]

oh yes, this evolved by accident no chance

[lolasexicans]

great job!

[sally12711]

thanks that really helped

[bluebirdHH]

very good video. thank you. but there is still something that I don't understand: the difference between positive and negative supercoiling.

[kapilpal1561988]

best video to explain supercoiling

[Shorty20122012]

nice. exactly what I needed. ;D

[RobotBadger]

EUREKA!

[richwaight]

great stuff!

[dpoet19]

excellent explanation!

[juliusangelo23]

beautiful

[bestrafe205]

this proces is before the helicase or at the same time?

[arpag]

i think it´s befour rhe helicase, and when the helicase arrives the topoisomerase goes/connects to the top of the helicase.

[abdul5900]

I think it's immediately after Helicase. Helicase has to first bind to the origin of replication and has to "unwind DNA, much like the guy who pulls the rope apart. Then you have the knotty problem as a result. That's when Topoisomerase comes in to relax coils.

[lMsoCOOL]

sorry, can someone tell me, if this happens in DNA replication? I have my GCE A levels coming right up and my teacher is next to useless, along with rude and fierce.

Thanks!

[draubrey]

Yes this happens in replication. When the helicase unwinds the helix it introduces the positive supercoils as shown in the video. Gyrase (a special topo II) is used to correct it by undo-ing the supercoils (by adding negative supercoils).

[lMsoCOOL]

thanks! :D

[barnequashesha06]

@lMsoCOOL yes it happens in dna replication

[juanwally]

thank for explaying me... From Chile :D

[Sh1kz]

thanks so much

[Sushili1985]

Thanks for making me understand this process :)

[pismacker]

Thank u for making it easy to understand...

[aalsayed89]

Thanks ever so much....

[spiritsA]

thanks :)

[youkoanika]

This vid is really helpful! Thank you so much...

[ioeopep]

This video really explains the mechanisms of topoisomerase!

Now I understand how they works !

Thank you for uploading this!!

[kiskka88]

fascinating