Action Potentials from Squid feat. Alan Hodgkin

[rahillatarfa]

omgosh why does it stop right when it's about to get even more interesting... I WANT MORE!!! :( Is there a more complete video somewhere???

[jwebster2469]

Those are some sweet sideburns...

[drskinnerve]

No, sorry, I don't

[looneytunes55]

do u have any videos on the reversal potential?

[drskinnerve]

I could imagine that you could try loading excitable cells with a potassium sensitive dye. With whole cell patch clamp you can then follow membrane potential changes while you change the potassium in the ECS and your imaging system follows changes of the intracellular potassium concentration.

Of course you need to calibrate your fluorescent signal first with different concentrations of potassium via the ICS in the patch pipette.

[VladimirMatveev]

Is it possible to study membrane potential - intracellular K+ content relationship by whole cell patch-clamp method?

[julienman328]

it would be hard, because the electrode solution itself contains K+.

[VladimirMatveev]

It's a great pity because the relationship is very important in the context of two competitive theories of cell physiology. My video about the theories you can find in my account.

[badger500]

One could use a whole cell pipette to load, over time (minutes) the intracellular cytosol with a solution containing no K+ (but corrected for osmolarity). One could also block various K+ channels with toxins, thereby subtracting their contribution from the resting membrane potential.