this is a little late, lol.. But the DNA seperated out because it does no disolve in the alcohol like the other stuff does. It's like oil and water. Oil does not dissolve in water and floats on top.
what happens if I try to look at those dna strings in a powerful ordenairy microscope? :P Will I see anything that look like a dna or just a thin layer of fibers?
DNA can be dissolved, it can be denatured into its constituent thingymagics (phosphate, base, deoxyribose sugar). Denaturation can occur from high temperature, high concentration alkaline solution, high concentration urea and high concentration of formamide
So, when dna is precipitated in alcohol, was it dissolved, or was it in it's original molecularly thin form, just not clumped with the other strands? Many thanks.
I believe that the positive charged Na ions bind to the negatively charged phosphate backbone of the DNA molecule, causing an opening of the secondary (and tertiary) structure. The atoms force open the molecule through steric hindrance, similar as to why all molecules have defined angles and shape. Also, they prevent the binding of positively charged histone proteins that help wind the DNA.
very helpful! I'm isolating and extracting DNA from mouse tails tomorrow and was looking for a visual/auditory aide, so this video was very helpful in understanding the step by step extraction of DNA. Thanks!
Great video. No doubt some schools now do this and I did it in university years ago. The simplicity of the extraction takes the abtract out of talking about DNA. A good way to spend a morning in the kitchen with your kids.
Such a nice video i ever watched!!
pushp01 2 years ago
AWSUM....!
SAJJJJJAD 2 years ago
Why did the DNA entered the alcohol layer????
dreamy2 2 years ago
this is a little late, lol.. But the DNA seperated out because it does no disolve in the alcohol like the other stuff does. It's like oil and water. Oil does not dissolve in water and floats on top.
nlgatewood 2 years ago
Its the same effect as oil on water
Oil is on top of the water.
DNA is the oil
Water is the alcohol layer
xtaxinyoface 2 years ago
I wonder if there is any pectin in that.
prayfertrey 2 years ago
Wonderful!
DawnFoxworth 2 years ago
what happens if I try to look at those dna strings in a powerful ordenairy microscope? :P Will I see anything that look like a dna or just a thin layer of fibers?
givemelols 2 years ago
DNA can be dissolved, it can be denatured into its constituent thingymagics (phosphate, base, deoxyribose sugar). Denaturation can occur from high temperature, high concentration alkaline solution, high concentration urea and high concentration of formamide
Melkor10 2 years ago
So, when dna is precipitated in alcohol, was it dissolved, or was it in it's original molecularly thin form, just not clumped with the other strands? Many thanks.
interstitialofficial 2 years ago
Of course DNA can only be seen with a powerful electron microscope but there is such a massive amount clustered together that it appears as a tissue
dreddythedragon 2 years ago
Thanks mate~!
5/5
binxbasilisk 2 years ago
Nice site 'n videos!
Cheers.
SweetFire96 2 years ago 3
you said alcohol will not disolve dna, just curious- what will disolve dna?
lejink 2 years ago
I believe that the positive charged Na ions bind to the negatively charged phosphate backbone of the DNA molecule, causing an opening of the secondary (and tertiary) structure. The atoms force open the molecule through steric hindrance, similar as to why all molecules have defined angles and shape. Also, they prevent the binding of positively charged histone proteins that help wind the DNA.
beeecraft 2 years ago 5
How do the ions "interact" with the dna to make it unwind?
interstitialofficial 2 years ago
Heh that's pretty cool. The DNA looks like a bunch of fibers.
opiates 2 years ago
that is a great video , many thanks !
alphadog7473 2 years ago 6
it's so cool that they teach the way to do it at home!!
It explained the process and theory quite well
sunnysided2 3 years ago
._. wow i thought the nuclius was the smallest it could get.but then it got smaller!I don't even know wat is a chromosome. 5/5 video!
Beardactal 3 years ago
can you look at it through a microscope O_O?
tyniehawk 3 years ago
=.= You'll need a powerful electronic microscope to see DNA
lemonsuave 2 years ago
thanks!
Zhangsiping 3 years ago
nice 3d action
mistermuffin420 3 years ago
I just looked at a website that said that on no account should I extract dna from animal cells, only plant cells. It didn't bother to say why.
prayfertrey 3 years ago
very helpful! I'm isolating and extracting DNA from mouse tails tomorrow and was looking for a visual/auditory aide, so this video was very helpful in understanding the step by step extraction of DNA. Thanks!
annakep 3 years ago
That was so cool! So, thats what DNA looks like! lol
zam626 4 years ago
Hila Road vids have got to be the best science for anyone type vids out there. Thanks! Looking forward to more.
jmcmovie 4 years ago 2
BRAVO!!!
YuroPula 4 years ago
five stars man thats awsome!!!
hondac55 4 years ago 2
hey nice video 5 stars
kj334 4 years ago 2
Five stars, another great video from Hila. Thank you so much, guys!
Battlefield2Pilot 4 years ago
excellent animations
laxguy1 4 years ago
excellent. Thank you!
invis648 4 years ago
Awesome. Thanks a lot for these videos, they're really interesting! :)
hamsteralliance 4 years ago
this is so cool!!! I am doing this in biology on Wed.
bea6993 4 years ago
looks like your teacher watches a lot of YouTube
hahaha
zetlaux 4 years ago
cool
oooorigin 4 years ago
Five star
Mikey34549 4 years ago
awsome vid!!
enderwigginsx 4 years ago
Great. The electrophoresis is one way to separate all the DNA and the proteins. The indirect way to know the proteins is to analyse only the weight.
Thanks.
biosisifo 4 years ago
Great video. No doubt some schools now do this and I did it in university years ago. The simplicity of the extraction takes the abtract out of talking about DNA. A good way to spend a morning in the kitchen with your kids.
pwarkentin2pc 4 years ago
why didn't we do such things at school?
YourMentalDisorder 4 years ago