hey could someone tell me whats the meaning of a negative operon?:) i know that lac operon is a negative inducible operon i understand the inducible part but keep getting confused about the negative part
@Microglia1 The lac operon is an example of prokaryotic (single-celled organisms such as bacteria) gene expression and prokaryotes do not have a double helix DNA structure.
This is not true, in the absence of repressor, transcription rates are still low. the gene needs to be activated be CAP-cAMP complex which bend the DNA. without this complex transcription occurs at a near basil level rate.
good video, but doesnt mention the other level of control involving glucose; the cAMP-CAP protein complex that binds to the promoter and induces transcription. This may be described on another video?! but just though i'd mention it
This is all true, except allolactose causes the confimational change in the inducer, not lactose. Allolactose is produced in the 2nd reaction of beta-galactosidase in which lactose is isomerised. This occurs when only residual levels of the enzyme are present.
@dreadyteddy really? i thought it was the repressor that allolactose binds to and makes it fall of the lacO site thereby allowing transcription to occur.
kann ich so nicht unterschreiben. (bin bio prof.)
DudeNumberTen 3 weeks ago
Very clear and succinct. Thanks!
abboyy 1 month ago
Comment removed
abboyy 1 month ago
hey could someone tell me whats the meaning of a negative operon?:) i know that lac operon is a negative inducible operon i understand the inducible part but keep getting confused about the negative part
stargirllilac 4 months ago
this is the best video on lac operon
ashishtewaricool 5 months ago 3
bota uma legenda filho
tatusnosuke28 8 months ago
wow this was awesome and helpful! yay for technology and visual videos :)
karissaXD 9 months ago
Think this is the video ever on youtube with no dislikes
chillifire 10 months ago
Why are the control units (operator, CAP, Promoter, etc.) all upstream to XYZ lac genes? Shouldn't they be downstream? since DNA is read 3->5?
Microglia1 11 months ago
@Microglia1 The lac operon is an example of prokaryotic (single-celled organisms such as bacteria) gene expression and prokaryotes do not have a double helix DNA structure.
123shala 3 months ago
good video, but unfortunately, it doesn't show the whole process. a real pity.
LilaBubble 1 year ago
great video!!! Teached me a lot!
Warphaker 1 year ago
This is not true, in the absence of repressor, transcription rates are still low. the gene needs to be activated be CAP-cAMP complex which bend the DNA. without this complex transcription occurs at a near basil level rate.
bigo195 1 year ago
well, lactose is changed to allolactose.
TheDeeqaluula 1 year ago
THANKS FOR MAKING/ UPLOADING THIS HELPFUL VIDEO
its saving tons of brain power which i need to read up on 4 more chapters before my final tomorrow! thanks
yea i definitely shouldn't have put my studies off till last minute again ;)
50kT 1 year ago 8
good video, but doesnt mention the other level of control involving glucose; the cAMP-CAP protein complex that binds to the promoter and induces transcription. This may be described on another video?! but just though i'd mention it
Charlee6227 1 year ago
This is all true, except allolactose causes the confimational change in the inducer, not lactose. Allolactose is produced in the 2nd reaction of beta-galactosidase in which lactose is isomerised. This occurs when only residual levels of the enzyme are present.
dreadyteddy 2 years ago 14
@dreadyteddy really? i thought it was the repressor that allolactose binds to and makes it fall of the lacO site thereby allowing transcription to occur.
V4nQuish1 3 weeks ago
buen video :)
lacri1000 2 years ago
great video!
gravyhauler49 2 years ago
Good, explain everything! Thanks
xleriaaan 2 years ago