Interesting concept!! But he succeeded by putting cDNA of polio genome in to cell free extract, which later codes for viral genome and made the infectious virus. But how he managed to get the RdRp of polio virus to transcribe the genome, as at initial he only put the cDNA with aneucleated cell free extract!! He could really failed,if he used viral RNA directly as template genome.
Interesting concept!! But he succeeded by putting cDNA of polio genome in to cell free extract, which later codes for viral genome and made the infectious virus. But how he managed to get the RdRp of polio virus to transcribe the genome, as at initial he only put the cDNA with aneucleated cell free extract!! He could really failed,if he used viral RNA directly as template genome.
phoenix88801 6 months ago
no shorts in the lab!
SteU4IA 10 months ago