Ah Chromatography, one of the greatest boons in Analytical and Organic chemistry. The history behind this technique is very sad. It's too bad Mikhail Tsvet was never around long enough to see his invention take the world of chemistry by storm.
I like that you showed the funnel underneath. MIT don't use one in their digital lab manuals. now have the sexy lab girl paint some of those on her and disco tech dance :P
Question for the chromo guys! If I've run TLC's and have a rough Rf value for compounds that I can't normally see under UV or without destructively staining, and I want to purify a gram, can I transfer the Rf with any degree of accuracy to a column with a similar packing? My plates are 60A silica, so 60A silica in a column, can I use similar elution times? There must be a known value for the sol. volume on the plate, which I could transfer to a similar column without collecting endless fractions
@Ryuusenka00 It's a piece of glass, plastic or aluminium that's coated in very, very fine, pure silica. The grains are tens or hundreds of atoms across, to create a super uniform, smooth surface ~1/4mm thick (like a filter paper, but better; I still use filter papers for rough tests before the TLCs). The glass ones can be tricky to cut by hand, even if you're used to snapping tiles for DIY (same trick). need gloves with all to avoid contamination.
c0kement0s again....very well done!!!!....to sum up, of course, I would say the separation was due to the polarity differences between the substances being separated. Indeed, there are different stationary phases (reverse phase, normal phase) thus 'interaction' with the stationary phase is more general and appropriate.
Brilliant! I have spent 3 years scouring YouTube for chem flicks to show my students. Your demos are superior - both visually and musically. Perhaps this 'Apex' company has taken over. If you haven't sold out your artistic genius, would you please consider now posting vids devoted to the 'BIG' principles: a)Temp & Rxn Rate; b)Conc & Rxn rate; c)Surf Area & Rxn Rate; d)Sythesis rxn; e)Decomp rxn; f)Single Displ rxn; g)Double Displ rxn; etc.
Your stuff has been the best I've seen in 3 years!
A BEAUTIFUL demonstration! Thanks for posting!
logicerr 1 week ago
magnificent video i was easily able to understand about chromatography thanks a lot
Arjun072 3 weeks ago
want more videos to explain hplc,hptlc,etc.......
saptarshisen007 2 months ago
awesome
saptarshisen007 2 months ago
чотко
chief1421 2 months ago
I hate this video cause I'm a pupil =)
KIENru 4 months ago
thanks dude
02abhicool 4 months ago
great video...what is the song i love it too!
TheAclark109 5 months ago
this is soooooo cool oo
karil15 8 months ago
fantastic! Thank you!!!!!!!!!!!!!!
enchantedMALE 8 months ago
that's cooool! :D i like the music too!!
chcolatteizzle 8 months ago
Ah Chromatography, one of the greatest boons in Analytical and Organic chemistry. The history behind this technique is very sad. It's too bad Mikhail Tsvet was never around long enough to see his invention take the world of chemistry by storm.
OfficeThug 8 months ago
this is the only video that has help thanx nnnnnnnnnnnneeeeeeeeeeeeeeeeeeeerrrrrrrrrrrrrrrrrrrrrrddddddddddddddddddddd
fishyroxxxx 9 months ago
Thank you!
wtfthu 9 months ago
This was a great explanation! The guitar was bit distracting, though.
missybebe74 1 year ago
amazing. best video in your channel =D
ThoseLatinoKids 1 year ago
thx
adoool777 1 year ago
very good video and easy to understand
mdogrnc420 1 year ago
I like that you showed the funnel underneath. MIT don't use one in their digital lab manuals. now have the sexy lab girl paint some of those on her and disco tech dance :P
lexichronicle2 1 year ago
Question for the chromo guys! If I've run TLC's and have a rough Rf value for compounds that I can't normally see under UV or without destructively staining, and I want to purify a gram, can I transfer the Rf with any degree of accuracy to a column with a similar packing? My plates are 60A silica, so 60A silica in a column, can I use similar elution times? There must be a known value for the sol. volume on the plate, which I could transfer to a similar column without collecting endless fractions
lexichronicle2 1 year ago
excellent, superb
Shehwazanwar 1 year ago
my teacher showed this in class. great n colourful demo. great music too.
ferdowzruqman 1 year ago
thanks alot
dinocar77 1 year ago
aww yeah great chem video, i love the lights display
Casablanca530 1 year ago 2
what is the TLC plate?what is it exactly?
Ryuusenka00 2 years ago
@Ryuusenka00 It's a piece of glass, plastic or aluminium that's coated in very, very fine, pure silica. The grains are tens or hundreds of atoms across, to create a super uniform, smooth surface ~1/4mm thick (like a filter paper, but better; I still use filter papers for rough tests before the TLCs). The glass ones can be tricky to cut by hand, even if you're used to snapping tiles for DIY (same trick). need gloves with all to avoid contamination.
lexichronicle2 1 year ago
c0kement0s again....very well done!!!!....to sum up, of course, I would say the separation was due to the polarity differences between the substances being separated. Indeed, there are different stationary phases (reverse phase, normal phase) thus 'interaction' with the stationary phase is more general and appropriate.
contraptionists 2 years ago
Brilliant! I have spent 3 years scouring YouTube for chem flicks to show my students. Your demos are superior - both visually and musically. Perhaps this 'Apex' company has taken over. If you haven't sold out your artistic genius, would you please consider now posting vids devoted to the 'BIG' principles: a)Temp & Rxn Rate; b)Conc & Rxn rate; c)Surf Area & Rxn Rate; d)Sythesis rxn; e)Decomp rxn; f)Single Displ rxn; g)Double Displ rxn; etc.
Your stuff has been the best I've seen in 3 years!
7517638 2 years ago 14
Now that, is awesome.
marine12320 3 years ago 18
What kind of liquid transfering devices are those? The one in your hand on the 16th second shot.
bluesephir75 3 years ago
a capillary tube
Limberger 3 years ago
This has been flagged as spam show
FIRST!!!!!!!!!!!!!!!!!!!!!
1trip711 3 years ago