great song! at 0:22, though, it looks like the image shown is actually of GFP fluorescing in the plasma membrane or cell wall - those jigsaw puzzle-like shapes are the cells on a leaf surface (you can even see some stomata in there) and if the fluorescence is in the tonoplast, then these cells appear to be literally ALL vacuole!
@PandasPet Thanks for your comment! I am copying in the reply from Dr Lorenzo Frigerio:
Unless you double-label with FM4-64 it's very difficult to tell plasmamembrane and tonoplast apart. In this case the telltale signs are the little transvacuolar strands that seem to protrude into the vacuolar void (there's a nice one at the center top, roughly before the 'g' of largest).
great song! at 0:22, though, it looks like the image shown is actually of GFP fluorescing in the plasma membrane or cell wall - those jigsaw puzzle-like shapes are the cells on a leaf surface (you can even see some stomata in there) and if the fluorescence is in the tonoplast, then these cells appear to be literally ALL vacuole!
PandasPet 5 days ago
@PandasPet Thanks for your comment! I am copying in the reply from Dr Lorenzo Frigerio:
Unless you double-label with FM4-64 it's very difficult to tell plasmamembrane and tonoplast apart. In this case the telltale signs are the little transvacuolar strands that seem to protrude into the vacuolar void (there's a nice one at the center top, roughly before the 'g' of largest).
plantendomembrane 5 days ago
@plantendomembrane cool! i had no idea that the vacuoles were so dominant in the pavement cells. i was hoping someone in the know would respond!
PandasPet 5 days ago
@PandasPet Glad we could help!
PS: Vacuoles are going to dominate the world (together with Golgi bodies, of course).
plantendomembrane 5 days ago