Dictyostelium cell migration

[seymoor]

And the magnification is?

[StevenKeirstead]

I can't really remember, since that movie was made a year ago. But it was probably heavily cropped from a capture with the 20X objective lens, so probably over 400X effective magnification in this video. The spot RT3 camera has a smaller field of view than you see through a 10X ocular lens, with a 2/3" (18mm) diagonal chip.

[pegasnegru]

What mutation did you make to the myosin? I mean was the effect conformational (harder for them to polymerize)? Sorry, I study translation in E. coli (lots of mutaions) but i was fascinated by your video.

[StevenKeirstead]

I didn't do it, someone at Harvard Medical School gave our course at the College the strains. The mutation is selected for with the antibiotic G418 Sulfate, so it must be a site directed insertion. Sorry, I don't know more.

[StevenKeirstead]

Note: I will remove all SPAM comments, including superstitious, chain-letter-type comments. This is a Science video, after all.

[StevenKeirstead]

This is shot through a Nikon inverted microscope through agar, which causes the graininess. The scope lenses are perfectly clean. Unfortunately, YouTube re-compresses the video which degrades the quality of the image. The uncompressed full-size version is much better looking.