Polymerase Chain Reaction (PCR)

[torktt]

i need this in french!

FRANÇAIS PEOPLE FROM CANADA!

[Benysaj13]

sweet bass line

[deepbit]

great video! Now I understand :D

[willrap4f00d]

and this is why youtube is awesome

[Number1JDAWG]

wow and suddenly pcr makes sense

[AmeliaThermopolis007]

I want it in PORTUGUESEEEEEEEEE! Some dedicated student could translate it!

Please!

[3385sandra]

very helpful, thanks

[grooshmonkey]

@darthawsome: you're right, I hate thinking too

[grooshmonkey]

Shouldn't the number of correct fragments be (N^2)-2N where N is the number of cycles? I think they frigged up on the 30 one, no?

[darthawsome]

@grooshmonkey Don't be a douche. It's just explaining the basic mechanics of PCR.

[moos209]

God bless you

[salemr86]

I wish they didnt include the awful music in the video. It only distracts and serves no purpose. Thanks for posting. Very helpful despite the music.

[maplecrisp]

@salemr86 I like the music.

[Robitybobitybobity]

too much bass

[DkanDywoot]

is it wrong if this video gave me a boner?

[ridamughal98]

grt vdeo,keep it up.......

[zzaccc15]

haha i was watching this and was thinking "i'll post a wanking comment cause it's a chick teaching me to do science"... *scrolls down*...

I'm so proud of you guys!

[fuzzb0y]

i found this difficult to masturbate to

[UlTrA2GaMeR]

@fuzzb0y #LOL

[nickd]

PCR always DTF!



[R6ngas]

Jizzed in my pants

[h2so4hno31]

@poltergeistx

it is not right...you need nucleotides=Desxoxynucleosid-t­riphosphates(Nucleotid=Nucleos­id+Phosphat)

as you know-the DNA cannot exist without phosphat!!!!!

[poltergeistx]

@h2so4hno31 why in heavens did I say that 0_o I feel ashamed lol

[thimwarrior]

she sounds HOTT

[jinal896]

got d topic 4 dissertatn....can nebody help????

[P3T3RYANG]

Heating, hybridization, DNA synthesis. Hooray for me! Lol.

[MaximumMaxime]

youtube helps and your video does too

[L30n3r1]

What happen to the longer products? Are they thrown away or kept in the mixtures? If they are thrown away, how to do that, and how you measure the final concentrate so that you are sure, that only the correct product remains?

[Mattprole]

@L30n3r1 I think the longer fragments are simply ignored, as they barely influence measurements.

[P3T3RYANG]

@L30n3r1 If you were listening, after a certain amount of cycles, you'll have such a substantial copied amount of the "wanted" DNA fragment that to choose the wrong sample to work with would be highly improbable. Measuring the final concentration, is probably explained else where, but for sure, not here. Also, knowing what you want can be further analyzed using Protein Electrophoresis, but that's a whole different concept. ;)

[542eastWA]

BEAUTIFUL

[Cali55nine]

I love the beat what's it called and where can I get it?

[pinflipoy]

Thanks so much for the upload. Things make much more sense now! :)

[YT092]

brilliant. puaha.

[nimeetgarg]

ITS FANTASTIC.

[cohen36]

k I would shove my dick up this girls fuckin clit

[bench175]

@cohen36 I think you don't know what a clit is.

[Moonshield15]

@cohen36 Hahaha, now that's gotta hurt.

[miglspam]

This video helped answer my questions about what happens to the original template and the "long" strands. Thanks.

[karlkarlkarl1234]

So is there like a formula?

(Typical exam question):

"How many correct copies of double stranded DNA containing only the target sequences are present at the end of the 35th PCR cycle?"

2^N (N = # of cycles) = total quantity DNA molecules present in mixture.

2N = # non-precise partially d/s DNA molecules.

So (2^N) - (2N) = # of ds DNA molecules precisely the length of the target sequence = # clones of the DNA sequence of interest.

Answer is then -> 3.44 * 10^10 copies.

Rock on bitches.

[Alxphat]

@karlkarlkarl1234 haha u made the shit so easy, i thought i was gonna study my ass off for this crap

[mrohith2010]

Excelent !

[quasarinstruments]

Great Video, very concise and easy to understand. Thanks,

[josenros]

Whoever came up with PCR is freakin' brilliant!

[Edensnator]

@josenros

I will tell my dad that you complimented him.

[flusete]

Guys, taq polymerase is the most commonly used but not the only enzyme that works in a PCR, that is why they simply mention a generic "DNA polymerase". This video is very easy to follow and I think is pretty much correct. Thanks so much for the explanation!

[sweetsweetdna]

if you want to amplify a specific gene, the primers are supposed to go on the outside of the target sequence.

[ethanhines]

O.K this makes sense to me for amplifying a piece of DNA but how does this process work for determine the amount of virus in a sample of blood? How can someone amplify the DNA and then determine the original amount? It doesn't make sense to me.

[DarkMetaRidley]

theres no mention of taq polymerase here!!!!

[SH3Z4D1]

@DarkMetaRidley yes thts true but she keeps on mentioning the dna polymerase, and i think only taq pol is actually used in pcr

[amounaamounaalgrie]

SVP di moi comment faire pour enregistrer ce video c urgent

merciiiii

[ethanhines]

@amounaamounaalgrie :télécharger le plugin "Download Helper" pour Firefox

[windowwatcher123]

Nice, but the value after 30cycles is wrong it needs to be 2^30-1-29-29-1=1.073.741.764

Correct me if I am wrong

[elmatto2006]

why read it when you can watch it? makes so much more sense! fuck!

[Kiwibro92]

@elmatto2006

Hey this is science. you shouldn't use such language

[elmatto2006]

@Kiwibro92 im mearly expressing my love for this video. it saved me in biology class.

[MissMetallica93]

AWESOME.

I hava an exam tomorrow and this thing SAVED ME-

thank you so much garlandscience!!! :D

[lafleur21]

well actually the correct sequence ans the target sequence are the same...cause the target sequence is the one you wanna amplify...and the correct one is also the one you wanna amplify...it's just to show you that the long sequences aren't just what you're looking for!

[ismaelsbaby]

great vid but i dont get it what do u mean by "correct sequence" and "Target sequence"? How do we know which is correct! HELP MEEE!!!

[mimiandolini]

Nt = 2 (n) x N0

[mimiandolini]

Little formula is necessary:

Number of DNA copies at n(th) cycle = 2 to the power n copies

[pablovox]

sexy

[donpadrementirosso]

Thank you very much

great work

[AlgerienBiologist]

thanks