DNA/RNA Transcription

[lmlm301]

where the promoter is located ?? is it on the template strand or the coding strand ? by logic it is located on the template strand and it must be at the begining of the strand which means near the 3' end while it is said to be located near the 5' end upstream to the structural genes ... it's really confusing

[geneedinc]

The noncoding/template/non-sense strand is used as a template for RNA synthesis. RNA polymerase traverses the template strand and uses base pairing complementarity with the DNA template to create RNA from 5' → 3'.

[geneedinc]

Although RNA polymerase traverses the template strand 3' → 5', the coding/non-template/sense strand is usually used as the reference point. So, transcription goes from 5' → 3', and the promoter is upsteam 10 to 35 base pairs (-10 to -35 bp) from the transcription initiation site (+1).

[geneedinc]

Eukaryotic polymerases do not directly recognize their core promoter sequences. RNA polymerase binds only after certain transcription factors are attached to the promoter. Therefore the DNA strands are still together (zipped) when these factors first recognize the promoter sequence. So describing the promoter as being on either the coding or template strand is irrelevant, since the promoter region (both strands together) is what is recognized by the transcription factors.

[lmlm301]

its too late , anyways thanx a million dear . I passed the exam successfully and i just realized how stupid my question was :D :D

[Qthepianokid]

Very basic but helpful. thank you!