You mutilated that brain. There's no way you can use it to get any decent immunohistochem done. But anyway thanks for a rough idea on how to do this :)
With this way you cant use this brain for sectioning. Their is another cleaner way to harvest a fully mouse brain without using Scissors or fine end forceps.
Also u need to perfuse this mouse to get blood-less brain.
Mice research has cured diseases for animals and humans alike. Too many ignorant comments. Hypocritical to say all these things yet use statins and pain medication.
On a side note, I'm pretty sure that brain cannot be used since you butchered it. I do electrophysiology and there is no way that those brain slices would be usable with all of the cuts in it.
@icarus999, I will seize your license if I knew you personally. It is not a right for humans to seize the right to life of other organisms! Call me mouse or names! I care for nature! You are just being selfish and I repeat, lazy. What do you mean you are the most adapted and most important organism on the planet. How are you important than the mice? Are they too not living in the same century like you? You are rather the most dangerous organism on earth today. I say STOP STOP STOP.
@Fwostyful, mankind is lazy and selfish. Selfish in deed! Can't you use yourselves for your works? Is it because "WE MICE" can not talk like you?, cannot fight you? cannot kill you too for our own experiment? We have done nothing wrong to you, leave us alone! Leave us alone! Do not confer the consequences of your negligence on us. We are animals. You are Humans. We mice are not the ones to pay the prize of cancer or what so ever! Leave us alone!
@GiNya4152 seeing as you seem to be under the delusion of being a rodent, you may wish to seek psychological evaluation. In addition, we will continue to utilize animals in medical trials and development, simply because it's cost effective, provides invaluable information, and to be blunt, because we can. Humans are simply more important.
@GiNya4152 Mankind is far from lazy. Humans are the most well adapted species on the planet. You're seriously deluded that animal testing is bad. Cosmetics are one thing. But when it comes to testing life-saving drugs it is completely justifiable. You need to take an anthropology class and learn a little bit more about ethics.
Looks awful! Stop using animals for human selfish reasons! What would have happened if YOU were swallowed by an animal? Would YOU not fight back? now what of this poor & helpless animal that cares for its young, fears dead, "reasons" like you? STOP STOP STOP
@GiNya4152 Selfish reasons? Humans use animals to experiment with drugs and potential cures for diseases that would debilitate people. The fact that you care more for a mouse than other humans is nothing short of despicable. I am an animal lover as well, but without animal testing there would be no cure for things like cancer, smallpox, or many other things you are inoculated for as a child. Do you know somebody who survived cancer because of a drug? This mouse is why.
@GiNya4152 Do you think if mice in our place they wont do research on use, would assume they would do more. Or do think its more convenience to experiment human brains from poor country? this for the advancement of human health and meanwhile understanding the animal health
@m76gmm I am from a poor country, so do you mean to use my brain? Should I hide myself from you? Well, leave the mice brain and come for mine if that is what you have been doing if and only if sacrificing mine will stop you from going to the second species of human you call poor countries. I will trace you out!
@GiNya4152 more than welcome, am also, imagine that their is a global pan on using animal in experimental research, what will be the alternative?
if ur tacking a medication very safely, do u think that came to u directly ,,, it got tested in many stages including animals so u tack it safely. What do u mean by tracing me out? is it a threatening?
@GiNya4152 heh, go a head, am civilized, I do respect human being and for this am conducting research to improve people live including u,, and using animals in research for advancement of the man kind.
By the way, who defend something he must got an alternative, have u got an alternative?
@m76gmm I will trace you to know how many brains you must have harvested from poor countries and you pay for them. Else explain what you mean by "experiment human brains from poor country".
Thank you for posting this video. I have protocols on how to remove the brain but a video is definitely worth more than a thousand words. Going to attempt to do this now.
@javiergonzalez94 Its either an INDIAN or an ASIAN.This is from mere observation. Secondly, people as this experimenter lack the ability to fell. It is a neurological disorder/ dissfunction. This is from studying neurosciences for 6 years.
You mutilated that brain. There's no way you can use it to get any decent immunohistochem done. But anyway thanks for a rough idea on how to do this :)
Neurogeekish 1 week ago
With this way you cant use this brain for sectioning. Their is another cleaner way to harvest a fully mouse brain without using Scissors or fine end forceps.
Also u need to perfuse this mouse to get blood-less brain.
Anyway, thanks for spreading science.
m76gmm 1 month ago
Mice research has cured diseases for animals and humans alike. Too many ignorant comments. Hypocritical to say all these things yet use statins and pain medication.
On a side note, I'm pretty sure that brain cannot be used since you butchered it. I do electrophysiology and there is no way that those brain slices would be usable with all of the cuts in it.
akaandy444 1 month ago 3
Good thing they did it on puppy pads :P
kaystepnick 2 months ago
what do you analyse if break every part of the brain? not cartainly Electrophysiological studies
GianluxRoccia 2 months ago
dam you!
TheJackabe 3 months ago
@icarus999, I will seize your license if I knew you personally. It is not a right for humans to seize the right to life of other organisms! Call me mouse or names! I care for nature! You are just being selfish and I repeat, lazy. What do you mean you are the most adapted and most important organism on the planet. How are you important than the mice? Are they too not living in the same century like you? You are rather the most dangerous organism on earth today. I say STOP STOP STOP.
GiNya4152 4 months ago
@GiNya4152 I would harvest your brain to figure out what we as humans shouldn't evolve to become. ;)
chaostroll1267 2 months ago
@Fwostyful, mankind is lazy and selfish. Selfish in deed! Can't you use yourselves for your works? Is it because "WE MICE" can not talk like you?, cannot fight you? cannot kill you too for our own experiment? We have done nothing wrong to you, leave us alone! Leave us alone! Do not confer the consequences of your negligence on us. We are animals. You are Humans. We mice are not the ones to pay the prize of cancer or what so ever! Leave us alone!
GiNya4152 4 months ago
@GiNya4152 seeing as you seem to be under the delusion of being a rodent, you may wish to seek psychological evaluation. In addition, we will continue to utilize animals in medical trials and development, simply because it's cost effective, provides invaluable information, and to be blunt, because we can. Humans are simply more important.
ProgressionEternity 4 months ago
@GiNya4152 Mankind is far from lazy. Humans are the most well adapted species on the planet. You're seriously deluded that animal testing is bad. Cosmetics are one thing. But when it comes to testing life-saving drugs it is completely justifiable. You need to take an anthropology class and learn a little bit more about ethics.
icarus999 4 months ago
just put mute and then play mambo 5 five
0B00BY0 5 months ago 7
Looks awful! Stop using animals for human selfish reasons! What would have happened if YOU were swallowed by an animal? Would YOU not fight back? now what of this poor & helpless animal that cares for its young, fears dead, "reasons" like you? STOP STOP STOP
GiNya4152 5 months ago
@GiNya4152 Selfish reasons? Humans use animals to experiment with drugs and potential cures for diseases that would debilitate people. The fact that you care more for a mouse than other humans is nothing short of despicable. I am an animal lover as well, but without animal testing there would be no cure for things like cancer, smallpox, or many other things you are inoculated for as a child. Do you know somebody who survived cancer because of a drug? This mouse is why.
Fwostyful 4 months ago
@GiNya4152 Do you think if mice in our place they wont do research on use, would assume they would do more. Or do think its more convenience to experiment human brains from poor country? this for the advancement of human health and meanwhile understanding the animal health
m76gmm 1 month ago
@m76gmm I am from a poor country, so do you mean to use my brain? Should I hide myself from you? Well, leave the mice brain and come for mine if that is what you have been doing if and only if sacrificing mine will stop you from going to the second species of human you call poor countries. I will trace you out!
GiNya4152 1 month ago
@GiNya4152 more than welcome, am also, imagine that their is a global pan on using animal in experimental research, what will be the alternative?
if ur tacking a medication very safely, do u think that came to u directly ,,, it got tested in many stages including animals so u tack it safely. What do u mean by tracing me out? is it a threatening?
m76gmm 1 month ago
Comment removed
GiNya4152 1 month ago
@GiNya4152 heh, go a head, am civilized, I do respect human being and for this am conducting research to improve people live including u,, and using animals in research for advancement of the man kind.
By the way, who defend something he must got an alternative, have u got an alternative?
m76gmm 1 month ago
@m76gmm I will trace you to know how many brains you must have harvested from poor countries and you pay for them. Else explain what you mean by "experiment human brains from poor country".
GiNya4152 1 month ago
Easiest way to get the mouse brain out is with a .177 pellet and a 1000 fps air rifle.
bigtruckseriesreview 6 months ago
Thank you for posting this video. I have protocols on how to remove the brain but a video is definitely worth more than a thousand words. Going to attempt to do this now.
TheEasyKill30 8 months ago
burp', i'm not used to see medical practice
sympasteve 9 months ago
I think the aim of such kind of operation is to study the relation between brain and mouse's behavior.
without brain, mouse can not remember anything, so interesting things will happen.
mackintosh828 10 months ago
the doctor put a band aid, the mouse went home and Silvester finally almost caught it
jradetzky 11 months ago
i think the mouse was dead...
alexhamster1134 1 year ago
what was the purpose of harvesting this brain? Are you looking for something specific. I'm just curious as to why it was removed so messy...
babyg412 1 year ago
... my worry is that you might turn people off of science/research because there is no context here.
darlantro 1 year ago
This has been flagged as spam show
You should really, REALLY describe how the subject was humanely euthanized,
... how this procedure was performed under strict animal care and use guidelines (don't rely on youtube'ers to look up what IACUC is),
.... and why this was done (how this particular research is valuable) in a concise and clear way.
darlantro 1 year ago 3
Comment removed
darlantro 1 year ago
WHY IN THE FUCK WOULD YOU UPLOAD THIS THIS IS HORRIBLE!!!! YOUR HEARTLESS...
javiergonzalez94 1 year ago
@javiergonzalez94 Its either an INDIAN or an ASIAN.This is from mere observation. Secondly, people as this experimenter lack the ability to fell. It is a neurological disorder/ dissfunction. This is from studying neurosciences for 6 years.
lty2k84 8 months ago
@lty2k84 wrote: "Secondly, people as this experimenter lack the ability to fell."
OMG! You mean they CAN'T cut down TREES? NOOOOOOOOO!!!!! The humanity! {:-(
;-)
Oh, and lying won't get you anywhere, and virtually your entire comment is a lie. :-)
ZachariasLePuertre 7 months ago
WHY DID U HAVE TO PUT SAD MUSIC ON I ALMOST CRIED TO DEATH
vequense 1 year ago 7
@vequense Why? Its dead, it didn't feel any pain.
FAB12UCB 4 months ago
@FAB12UCB i knew it was dead, but that song is more creepy now rather than sad to me ............
vequense 1 month ago
@vequense technically its mystery music
imeegz 1 month ago
@imeegz i don't even remember postin gthat comment though
i'm not one of those sensitive people ;)
vequense 1 month ago
Comment removed
jamurphy21 1 year ago
i wont do that to my own mice ..i like keep them alive better.
limdaf 1 year ago
thanks for uploading this, but better if you use a tripod or somehow keep the camer still.
galenicr 1 year ago
I guess you missed the Liquid Nitrogen container next to the mice!
Protitee1 2 years ago
Comment removed
pieceofmind616 2 years ago
eeww
TheAlecB123 2 years ago