Plasmid Cloning

[thomasthetruck]

I think it is physically impossible for his voice to be any more monotone.

[EcKoSniping]

I turned it off cause his voice had no excitement and gave me the idea of killing myself

[TheGmThunder]

Thank YOU !!

[jonaboy3]

Very good video, the audio really sucked tho =/ (couldn't add it to my presentation)

[ilir124]

thnx to mutch ......



[TheEthnikman]

thank you for posting

[sultanofebla]

Easy to follow

[janetulips]

Good Video for beginners.......

[jay8950]

i got an A+ in molecular biology thanx to youtube :D

[arvininfinity2]

Nahh ..gud job 

[Eszterlanc01]

E. coli must think we are god. :) We gave them miracle, survival.

[thewarriorforce]

so you can MAKE plasmids??? :D

[timster112]

@thewarriorforce It still is more of a modifiing act rather than creation :P But plasmids for humans are going to be a lot more difficult and I dont think I'll be lighting my smokes with the tip of my finger any time soon lol

[thewarriorforce]

@timster112 heh I wanted to fry someone but oh well

[ZedeAdHD]

gaattc aka im confused

[cadman7658]

good video！！！！！！！！！！！wow！！！

[cadman7658]

good video！

[tiger1227ja]

that wa a good video

i

[50kT]

too bad my teacher didnt tell us to study this...... i woulda gotten those 12 pts.....

[ghetto0superstar]

dude that was crazy good

[Jesusandashley]

Thank you soooooo much ! i have to do a FLR on this and now im pretty sure im getting a freaking A+ :D

Thankssss<3

[DollWreck]

YESSS. Thank you so much for posting this video. Very descriptive

[alexmetallicaZFC13]

stops there? i wanna see how do we choose the appropriate colony!

[SupermanTheOnly]

@alexmetallicaZFC13 I think once you've got all those colonies geographicaly separated, you have to put them in individual culture and then identify the right colony by any process. For example with an labeled hybridation fragment you can show on autoradiography ; if you get a radioactive signal, you can tell it's the good colony. But that's only my opinion... (excuse my approximative english :D hope you understood it).

[crazyguyonthekorner]

MOTHERFUCK!! I thought that this was a badass plasmid for bioshock

[Catsinabirdcage]

@crazyguyonthekorner What's Bioshock?

-Sir Joshua

[TheBlackParadeHasYou]

@Catsinabirdcage It's a game.

[timster112]

@Catsinabirdcage Videogame in wich you can enrich yourself with 'plasmids' to give yourself powers like fireball throwing or freezing or lightnig etc.. Hard fiction but really awesome :)

[PlutonGB]

this was WAY BETTER than reading 3 pages of a book

[snuckles108]

I just need a few DNA of Selena Gomez and then I get to have my own baby just like Selena

[PedroSSMferrari]

ok Andrew Ryan, now make it trow shockwaves in crazy zoombies (would you kindly?)

[AxisMundiOnline]

Thankyou for this.

[liho123]

thanks

[CONCUSSION187]

pretty complicated

[beanieboy911]

wow, i am 6 grade and i find this very interesting

[jeeman100]

bioshock? XD

[Nayyab5]

how calciium chloride aloow the recombinent DNA to enter in the e.coli bacteria.?

[liangjackson]

@Nayyab5 CaCl2 (calcium chloride) will strip off the proteins on the membrane of e. coli and make the cell "competent", or able to take up extracellular DNA.

Another method of making e. coli take in DNA is using electroporation, where electric shocks are used to introduce pores in the membrane long enough to introduce DNA molecules before the pores close back up.

[superheronumber1]

@liangjackson I am curious, doesn't treating e. coli with calcium chloride which strips off membrane proteins have some sort of deleterious effects on the bacteria? These membrane proteins have functions so stripping them away would have some detrimental effect on the organism. Does anyone take that into account?

[Yazzarh]

I was brought here by Dr Rowland from the BIOC 2000 course at UQ.

[doogiedog1234]

@Yazzarh me to i have my prac tomoro!!

[Yazzarh]

@doogiedog1234

Hope you did well then!

The subject's not *too* bad, on the whole.

[foeasy16]

@Yazzarh

not everybody goes to "UQ" so keep ur abbreviations to urself or spell it out

[Yazzarh]

@foeasy16

Erm...I don't have to, nor am I obliged to, and so I won't. Who says that you're allowed to tell others what they can and can't do on the internet, or what they should or shouldn't do? Because I'm not going to be doing what you say in this situation. Nor should I be expected to.

Haven't you ever heard the saying of "If it doesn't concern you, stay out of it".  You don't have the self-righteous, narcisstic claim or right to have to know everything that's going on with everyone.

[Highway420Music]

Politics, money, war, death, religion, freedom, 420, 9/11, 2012, Planet X, what? huh? Maybe it'll come together for you in my new music video, maybe not. You tell me. The video will be my comment. To see it for yourself, click on my user name or search youtube for "The Urban Legend of Planet X" or "Highway420Music" Please leave a comment. Send it to your friends. HELP ME MAKE THIS THING GO VIRAL! thanks thc

[mfrekerara]

This was very helpful, the animation was easy to follow with the narration. THANKS!

[anyee123]

thanks hope tomorrow exam i can answer well :)

[iVuHh]

great.. i understood it better!!! super thank you!

[3700956patrice]

super helpful

[proteinP53]

very good...thanks 5/5

Muy bueno gracias van 5 estrellas

5/5

[yarden123]

extremely helpful!

thanks a bunch.

[Jtran84]

Nicely done. So easy to understand. Helpful with my mcat biology. Thanks!

[l0vef00l0s0pher]

hi sorry for my ignorance but i have a few question.

i'm having trouble understanding what the point of plasmid cloning exactly is, which is making reading the course material extremely difficult.

when you talk about cloning do you mean that of the bacteria? is this how virus or bacteria grow. i guess what i want to know is what is getting cloned and for what reason.

again sorry..i'm just having a lot of trouble understanding.

[SlightlyVisible]

That's why UWI is a great university.

They actually care that students understand.

This entire process is caled molecular cloning. The purpose of which is to clone DNA (not entire DNA strands as they as too large but fragments).

[maradona4u0]

Hello,

Yes Plasmids are used to be a vector for a certain DNA mullecular, Which is wanted to be doubled and again like this. Generally, the cloning is being made in order to enrich DNA and cell researches.

Hope I helped.

[WolYou]

Nice! E. Coli! The guys growing in your asshole.

[w1nd12]

wolyou ur gay

[betthisonesfree]

now all i need is a cow and Barbara Streisand...

[SPINEBLOOD555]

what this got to do with bioshock ... oh it dont

[Sandgaara12]

I thought this was Bioshock...

[RFT15ify]

@Sandgaara12

Same here!

[MissiKickAss]

@Sandgaara12 ...bioshock?..i dont get it..

[PedroSSMferrari]

@MissiKickAss the game! search on youtube!

[MrBuBbL3s54]

@Sandgaara12 it is in a way the creater of bioshock used many facts of real science

[Makedonche]

@MrBuBbL3s54 They would have, like the author of Jurassic Park got science inspirations for his novel.

[117Mastermatt]

no wonder why jack was scearimg on bio because bood was exploding inside him 0_0

[chhotu007]

molecular biology at its finest...i love it

[magiccatalyst]

brings back memories of bio 104. Thanks.

[afawki]

anyone knows how can i get the full series such videos.....

[skyuppercutt]

woooooow. Thank you sooooo much..

the video is reallly good. it really helped me understand what i need to know.

THANKS KEEP IT UP :)

[dogoncouch]

rhs is for nubcakes

[jcesar810619]

look SourSweetSoul2, por si no conoces este es un metodo para AMPLIFICAR secuencias de ADN, asi como se hace con la PCR

[SourSweetSoul2]

Why is there multiple DNA fragments in the tube?

The gene we isolated by EcoR1 doesn't provide us with the specific DNA fragment that we need?

Why do we have many different DNA fragments in different bacterial colonies? and How is this problem solved?

I know it's a stupid Q.. :P

but i really wanna understand this bit :(

[elunicodeivyn]

Exelente video.. Saludos desde Ecuador

Att. Deivyn

[freescorfy]

Very informative!!

[kevin4free]

4:13

[ChaotiX64]

Really good. You should mention that EcoRI is one of many RE's. Also, treating E.coli alone is often not enough to make it competent to pick up a plasmid. Heat-shock is then used.

[Dizcinger]

Thanks

[JoeNoobie]

now you're thinking with plasmids

[Metaldude1945]

What can we actually benefit from cloning?

[legatoJR]

One example is plasmid cloning of human insulin genes in bacteria, and these bacteria can then produce human insulin. The insulin can then be harvested for use in diabetic patients.

[Metaldude1945]

Cool, go cloning!

[TooSmallToBeTrue]

Like insulin? . . . but what if your body rejects it?, also can you use this method to geneticly engineer plants such as yeast and sweet peas. . .

[legatoJR]

Human insulin from plasmid cloning is not rejected by human bodies (usually), as opposed to bovine or pig insulin (we used to use bovine and pig insulin a lot).

Just to clarify, yeast isn't a plant, but a fungi (don't have chloroplast and can't photosynthesize). You can use plasmid cloning in yeast vectors, their called Yeast Artifical Chromosomes. For plants, you use other methods, than this, such as Ti plasmids, particle bombardment, or microinjection.

Source: Principles of Genetics 4th Ed

[TooSmallToBeTrue]

ahh my bad :P!, thanks anyway :D

[lilgogeta]

cool stuff man I did that at school xD

[alephjajs]

Thanks for this

Where is the rest?

lpB

[Noahfreakinrules]

huh?

[teengag]

Do you have any references?

[BloodxGusher]

Hella Lost

[FasterSoldiers]

lol, for real

[AnatomaniaK]

so two birds with a single stone. cloning DNA and ampicillin resistance. actually 3 because you now have only inoculated bacteria.

[leprinceaxel]

thanxxxxxxxxxxxxx

[cradleofjohannes]

what a self explanatory video

[minh8001]

Great!

[alinmimi]

do you have the rest? the separation?

[AnotherSexyLoli]

Whats this for man?