Nanodrop it like it's hot! Nanodrop - Brown iGEM

[alexcastillo22]

stupid tung clicks

[ThePatrickb33]

hahahahahahhaahahhaa... the ending tune

[christoph06BERLIN]

awesome :D

[toxicmegacolon]

It's great if you can nanodrop $100,000 on a spectrophotometer.

[cbernier3]

@toxicmegacolon they are less than 10k, probably only like 5k for a university lab. that isn't much. Do you know how much we spend on just chemicals every month? Science isn't cheap.

[buuuuhahaha]

nerds

[darksage29]

The only thing I don't like about too much automation (although I do note that that thing really is amazing) is the fact that, computing for the concentration using spec is what all students are taught, and for some reason, automating the process could make the operator's brains to rot... Ahaha. I'm just old school that way.

[pGFTuv]

hey, that's the nanodrop my univ has! :D

[3j0a67bk]

Good advertisement... it´s like those ones presented by Mike Biceps and MarY Abdominals to sell the new "Press-Ups 3000"...shabby, shabby

[caaammmz]

i literally just dont understand what the fuck is going on

[wherewillyoube]

You won't believe this but my lecturer actually showed it during our lecture. Now that's awesome :D of course we had a hearty laugh at the final scene! Teehee!

[Rsavage08]

What is the song in this around time index 2.47?

[bendhoward]

Porcelain by Moby

[xXTheGreatestOneXx]

nerds!!! Gah!!

[livking]

post two papers to Nature! in 20 minutes...some kind of genius

[RedDaVincy]

With the NanoDrop (tm) spectrophotometer, anytthing is possible!

[FranzEhr]

@livking Who said they got accepted or even past review :-)

with 2 papers in 20 minutes his literature was probably mickey mouse :-)

still 20 min is at least good for a coffee....

[delawarerocks]

This video is a total turn-on: the nanodrop with Snoop Dogg's song in the background...BRILLIANT.

[Targaeryen]

Yeah, now I want a Nanodrop poster above my bed

[BodaciousBurnley]

haha. nice ole-timey treatment of the original spec. a great video, guys!

[illZwhY]

gosH i cannot blive thiz vid is kinda interesting...

[meowludo]

rofl. pie-pet it back up.

[Miyiku]

OMG WANT!

[cghmaster]

Beware of very high molecular weight DNA or your Nanodrop will end up in the parking lot with a trail of Lab debris behind it!!!

[SpectatorAbExtra]

Oh my!

I really want one now...

[qmwnebrtv]

OH i love the nanodrop! i love the clicky sounds the lever makes at work.

and yes. super easy to use.

[stepb24]

I love the clicky sound too! Haha.

[stlunaticajv]

i caught up on my literature...AND SUBMITTED TWO PAPERS TO NATURE!!!! WHAT HAVE YOU BEEN DOING?!?!?!?!?!?!?!

[microzoo]

this guy can catch up on his reading AND submit TWO papers to Nature, all in 20 mins?!? He is a machine as well hmmmmmm?

Jamie can do Maths AND probably he went for a coffee with friends, keeping a social life as well as working....

[Ironduke666]

he totally ignored the blank-mesurement

[meowludo]

You totally ignored the dictionary. lrn2spell

[binkari]

I kept waiting for something to terrible to happen with that FLAMING BUNSEN BURNER.

[ChemistryGirl09]

Wait a minute... if it's accurate to "2 nanograms per microliter", why is he reporting a "76.4 nanograms per microliter"? The decimal isn't significant at all!

[DrunkenCellist]

2 ng ± 2% error, so he'd report it as 76.4 ± something where the 0.4 is still significant, but contains the uncertainty.

[serpentcharmer2006]

We have one at work and when measuring different samples from the same source the results vary considerably... we've gone back to the old way... waste of money

[magstardrums]

@serpentcharmer2006 you probably had a shit sample, aggregate DNA...

[RailmanDave]

wow, that's an awesome machine. i've done it the old school way as well with a spectrophotometer and that nanodrop seems so much easier.

[bobjonesu]

too bad its like 8 grand :(

[Iosif53]

Oh my days.

[millandudu]

One question:

Why default wavelength is at 230 nm, if absorvance of nucleic acids is better at 260 nm?

[hirokury]

que mide l concentracion de proteinas y su pureza estupìdo

[millandudu]

hirokury,

You don't understand me! And I don't need your help!

Dumb!!!

[1Messiah4All]

sorry for late answer,

but if I'm not mistaken proteins will also absorb at 260. Cause of some aminoacids.

230 should avoid this...

Hope I'm correct and you are helped out

greetings

[millandudu]

Thanks!

[mimiandolini]

the ratio 260/230 must be > than 2. it assess the presence of contaminants wich absorbs in 230nm

carbohydrates and phenol all have absorbance near 230 nm. The TRIzol reagent (for RNA extraction) is a phenolic solution which absorbs in the UV both at 230 nm and ~270 nm

[nhojmabon]

This reminds me of 'The PCR song by Scientists for Better PCR'. Now that was a good advertisement!

[sharfee]

nanodrop makes life soo ezzzz.

[nduguchansler]

I CAN'T HANDLE THE NERDYNESS

JESUS

[MuppetMower13]

one question: can you make 70k straight out of school?

[sesemeoil49]

hahahah cute ^^

[semandoogon]

very bad intro should have started with potential benifits vs bad aspects informally, then the rst but by the end i had no doubt of the massive superiorty of the nano dohicky and if i had or worked in a lab i'd pay the price for acuracy and time saving and enhanced modes of use

[dougbrummell]

"In that time (25 min) I've managed to catch up on my literature for the week and submit two papers to Nature."

Haha. I wish.

[bam22056]

"siiense iz too confuzing for mehhhhh..."

these guys have probably saved your life more times than you can count.

[ericalexander19]

I wish that the end and the begining were the only parts of that movie

[mastershake1000]

WTF?!?!?!?! I DIDNt UNDERSTAND SHIT!!!!!!

but wow. i wonder what scientist are trying to accomplish?

why cant we live in a civilized place... with science <- BUT NOT TOO MUCH!

[Aaberg123]

You live the way you live due to scientists, so I don't see how you can have too much science. (Science literally means knowledge, and who the fuck want to be ignorant?).

[cruxbucket]

lol civilization started because of agriculture. which its self was learned through science. MORE SCIENCE ALL around

[willpu]

DORKS!!!!

[umitonami]

geeky...

[kenn0285]

I used the nanodrop in college...it is pretty cool.

[csimo13]

i nanodropped your mom!

[LabSnap]

Love this video! Now that NanoDrop is part of Thermo Fisher their 2nd gen products should be very interesting. I've seen their high throughput model, but not played with it yet. This model in the video was a real game changer though.

[Ipikop]

lol @ the two papers to nature

[Villentretmerth]

those "no life" dudes make sure you don't die next time you wind up in a hospital. Anyways, it's a much more productive way of spending time than watching promoted videos on YouTube

[LeaM87]

OMG! WE NEED THIS IN OUR LAB. it frusterates me when ONE sample is off.

[paulajayperry]

tee hee hee

[drosophila82]

awesome piece of equipment

very gay couple

[ITHEREONETHATHASNT]

Isn't there a concern about cross contamination. Just joking! I don't have a damn clue what you're talking about.

[xtothel]

Neeeeeeeeeeeeeeeeeeeeeeeeeerds­

[UncleRico13]

This seems way easier than the spectrophotometer we use. What's the catch. I see below it says it only cost around 5500 which is what we paid for ours.

[fleek164]

post 2 papers to Nature...hahaa

[dixonmciver]

Brilliant!!!! This video has made a very boring day at work (in the lab) enjoyable. Rock on guys!!!

[boysgo]

we are using this type in our lab and its pretty easy and smart. we have good practise with RNAs but we are not successful with Lowrys. does anybody know how to measure proteins on this?

[kofkok]

How much does this thing cost? I just need a ballpark number please.

[crizman88]

It's around $5500 per unit for the ND-1000 (The one you see in the video.)

[Novaspec]

For you low level guys and gals then try the NanoVue from GE - its easier, faster and looks the part.

[kcoldie]

sponsored by NanoDrop! No way! What you fail to mention is that 1 µL is not usually a suffiencient volume and the Nanodrop tends to need 2 µL to teake real measurements. Although all of the advertising material says 1 µL will do. Jibbed! Nevertheless our NanoDrop has been nominates for the greatest timesaving device in our department.

[xJuicyCoutureLuvrX]

lol thtz hilarious! i watched it w/ my dad nd sister nd they lovedd it 2! awesome job u guyzz

- chandler wessel

[donaldwtube]

John, that's fantastic. Good to see you again!

[dnajam]

Great Work John, good switch..

--danishnajam

[dugong13]

That video is nanodropalicious!!

[kielhoff]

Great piece of work!

[rahulahu]

Niicceeeee....

[DaveShi]

ahaha

[gregatpierce]

I like pizza!!!