I think you guys should keep in mind that this video appears to be directed at highschool students studying the lac operon, and therefore only covers the basic process. In my highschool textbook, they explain the process as lactose binding directly to the repressor.
the inducer DOES bind to the repressor. Hence, this changes the conformation of the repressor not allowing the repressor to bind to the DNA. Thus, the RNA polymerase is now able to transcribe RNA and the operon is expressed.
@409Inverted well if you really want to get technical the video also forgot to mention about the CAP - cAMP complex which allows RNA polymerase to recognize the mRNA strand and start translating. When glucose is present it inhibits acetyl colase (which produces cAMP from ATP) so no cAMP is made, CAP - cAMP can't bind so no gene expression. If glucose is absent, lactose abundant, cAMP is produced, CAP - cAMP bind to promoter, RNA polymerase binds, lactose binds to repressor, translation occurs
I think you guys should keep in mind that this video appears to be directed at highschool students studying the lac operon, and therefore only covers the basic process. In my highschool textbook, they explain the process as lactose binding directly to the repressor.
purple0381 3 months ago
what about cAMP and glucose levels? They play a major role in the lac operon, and it's not discussed
maxschell 4 months ago
piece of crap - it only talks about the first lock (the repressor) and not the major lock which is disengaged by cAMP binding to CAP.
epi8 6 months ago
Good video, although there are some major errors in it ^^
spazmbazm 1 year ago
yea i did found it weird he says lactose binds to the repressor. i mean wtf. get your facts straight.
Leetuss 1 year ago
cheers very helpful
splendourful 2 years ago
very good vid. thanks
MsDamon 2 years ago
the inducer DOES bind to the repressor. Hence, this changes the conformation of the repressor not allowing the repressor to bind to the DNA. Thus, the RNA polymerase is now able to transcribe RNA and the operon is expressed.
agnes213 2 years ago 4
nice, but......LACTOSE DOSE NOT BIND TO REPRESSOR! Some small amounts of lactose are converted into its isomer, allolactose, which binds.
409Inverted 3 years ago 12
good point... the fact that the isomer induces (and not lactose) was stressed in my lecture
anteater2011 2 years ago
@409Inverted well if you really want to get technical the video also forgot to mention about the CAP - cAMP complex which allows RNA polymerase to recognize the mRNA strand and start translating. When glucose is present it inhibits acetyl colase (which produces cAMP from ATP) so no cAMP is made, CAP - cAMP can't bind so no gene expression. If glucose is absent, lactose abundant, cAMP is produced, CAP - cAMP bind to promoter, RNA polymerase binds, lactose binds to repressor, translation occurs
ojoe4 1 year ago
@ojoe4
23crazy23 1 year ago
Very F@cking nice work!Thanks a lot :D
ulv3r 3 years ago
OMG! who ever did this video is a genius...I've been trying to understand the lac operon for hours!!!! Thanks so much, it really helps a lot :-D
plamenovaO 3 years ago
Great!Internet rules!education for all!
leonidaskavakos 3 years ago
thank you so much for this! amazing video and all the details!
cutiestar300 3 years ago
dynamic models r the best
robertoop 3 years ago