Restriction Enzyme EcoR1

[JejeBabe]

that sounded like a horror rape movie but good video

[MBmrBIGstuff]

Educational, but kinda creepy...

[q0w1e2r3t4y5]

i cried

[155ZAZA]

My friend was right, this is scary...



[FIEKESAYSHELLO]

I've learned that those Restr.-enzymes are necessary for Southern Blot Analysis... now I know how those enzymes work... but I still don't get the use for Soutern Blot Analysis.. So please help (:

[leonasmile]

*hear*

[leonasmile]

this is like a horror movie..here the sound...creepy

[devmanboarder990]

that was terrifying



[GJEViLakaTHEKING]

Here is my Commentary

it is a dna molecule.

at 13th second, the blue coloured being is known as a restriction enzyme. these are enzymes that find a specific site on teh dna molecule and cut it. It makes staggered ends.cutting process requires a Mg molecule and it produces 5' PO4 end and a 3'OH end.

this end is recognized by a Ligase. this is an enzyme that can join the two ends togather. two of them are needed for two strands and thus we have successfully placed a dna fragment into a plasmid.

[Kristen1641]

This is super scary ... and it kind of grossed me out for some reason...

[ItsARachel214]

That was so scary.

[TheRealZen00]

If I could get a copy of this I'd love to add commentary.

[lyndis10]

cutieeeee enzyme

[gopuishere]

really gud video

[hober184]

Is beatiful... now understading to restriction ezymes

[gabegw1]

that was the scariest restriction digest I have ever seen

[DanielHHowell]

This is the greatest and cutest enzyme of them all!

[sadamnixamani]

Yahans maa khe kare chha tho!

[PacoTheMuffinman]

...nightmares

[kevdndri]

What you see here at first, is the restriction enzyme ECOR1 searching for its recognition site on DNA. Once ECO-R1 has found its restriction site it will cut the DNA with the creation of the so-called "sticky ends". Now other DNA that is also cut with ECO-R1 will fit, due to initial stabilisation of H-bonding. What you see in the end is the enzyme DNA-ligase that will covalently bond the two DNA strands ...

[ariabm44]

the enzymes are really cute!:)

[jfoges4]

that was cool



[jonahfox]

Wow thats crazy.

[ghetto0superstar]

sounds like a night in the jungle

[khy03]

I clicked on this to learn, not to shit my pants. Holy shit.

[ladiieemayy]

@khy03 lmao fucking hilarious

[ExpertOfSound]

it is a super fast rollercoaster of death.

[NuttaPearl]

Cool animation!

[misantropaable]

... the sound is SCARY!!!

[uncustomized]

i think this is a very awesome music video

[miza310]

well that sucked

[burlappsack]

so the overhangs just fly right into eachother seconds after cleavage? i think this video fails to explain the chaotic nature of brownian motion, but is helpful none the less

[nalinirau]

that was extremely helpful

[panesar1987]

the geen one is called DNA ligase, which joins the DNA strands.

[wendijoyce]

the pink one is the vector? cleaved by the blue one, which is the restriction enzyme? the green one, which binds the new DNA fragment to the vector is called??

:) thanks, nice animation still.

[nalinirau]

@wendijoyce

dna ligase=green one.

yup, the rest youre right (:

[PrivateHeaven]

Not until I've seen ur comment I was like this representation is wrong the Restriction Enzyme EcoR1 doesn't join them together ...Ligase does that job.. & reading ur comment I saw u made the difference in colors & I was able to see that ligase was indeed represented...//

The title threw me off the title should have been Restriction enzyme EcoR1 and ligase

[ttlyADDICTED2BASS]

A bit of commentary would be nice...

[dbeecher]

@ttlyADDICTED2BASS Here's a longer and commentated version of this video: /watch?v=8rXizmLjegI



[brit387]

@dbeecher thanx

[ange53072]

That's nice.