Lec 15 | MIT 7.012 Introduction to Biology, Fall 2004

[TXRebelOK]

How come the cutting enzyme doesn't cut in the middle of a gene?

I.e. why doesn't the purified bacteria just a hole bunch of bits and pieces of fragmented genes and not a entire working gene as he states?

[sarshoy]

@TXRebelOK You're right, the bacterial restriction enzyme does not discriminate between ends of genes and sections within genes. But for the purpose of what the prof is trying to accomplish (identify a specific gene), this does not really matter.

[pg1033]

@TXRebelOK i think dats bcoz d enzyme recognizes d recognition sequence...

[VoteNixon2008]

Could EcoR1 be in there to cut the DNA for the replication of the topoisomeric plasmid?

[cchhrriiss825]

i learned some of this in iving enviornment.. woo

[661966]

this prof is good....nice explanations

[BluePaintedChair]

wow using bacteria to copy and paste human dna so that's how its done.

[dirkthedaring71]

A fantastic teacher which is unlike the vast majority of university lecturers in the U.K at least.

[purplepolygons]

This guy has taught me all the bio I'm supposed to have learned in my course