microRNA formation and function

[Szklana147]

follow the schema (step by step): wikipedia*org/wiki/File:MiRNA-­biogenesis.jpg when you are watching that animation.

[blackhawkVA1996]

It is very nice,It would be better if each step was explained.

[mp22012]

Very nice video - can I please have a copy to show to my students?

Thanks

[Sarmastani]

It is very informative. It would be better if every step was explained verbally.

[shneader1]

The inhibitory RNA is not ssRNA; it is an antagomiR, like LNA or 2'O-Me. It can be delivered directly to cells.

In mammals, generally miRNA-loaded RISCs don't proceed through Slicer mechanism to amplify the inhibitory response. Only in lower organisms.

The yellow thing is RISC.

Wished they would distinguished pri-, pre-, duplex, and mature miRNA. Also, where's Drosha/DGCR8? I guess good enough for lay people.

[chenalos]

This is fairly accurate but it doesn't show miRNA's ability to influence Slicer and Dicer to break up the mRNA, as well as amplify the inhibitory response.

[xhanlex]

Why there is no sound on this Vids? Anybody can help?

[KyaliApps]

Anything on inhibitory RNA

[Jesusolmos1990]

what's the yellow thing? 0:44

[scientificallyunstab]

that is the argonaute protein, an endonuclease that binds to the microRNA and causes part of its function, the degradation of the messenger RNA. There are also other proteins that help in the mRNA degradation/inhibition of protein translation, such as TRBP and Dicer itself (which cleaves the hairpin precursor into the mature microRNA) and together with the argonaute (aka Ago) they form the RISC complex (RNA induced silencing complex) that is in charge of doing those functions.

[bedabug0]

Where can I find a video that actually shows me how to make and use miRNA though?

[SSTTEEAALLTTHH]

You are going to make and use miRNA? good luck with that :)

First of all, miRNA is made by the cell itself. It will not work if you inject a short single strand of RNA (Fire and Melo tried it). For RNAi to be exogenous induced, you need to inject dsRNA

[scientificallyunstab]

ah vale, me acabo de dar cuenta que eres español...bueno si quieres que te diga lo mismo en español avisa oiste ;)

tambien si necesitas saber algo mas pregunta que te puedo decir bastante sobre los microRNAs (acabo de hacer una rotacion de 3 meses en un laboratorio de microRNA, asi que lo tengo bastante fresco)

venga!

[SSTTEEAALLTTHH]

@Jesusolmos

That's the Dicer-siRNA complex i think

[breastplates]

looks like a loaf of bread to me :D

[FenderMike1428]

waaaaaaaaaaaay over simplified

[cutecloner]

I agree....much too simplifed but the animations r gr8

[jaxford25]

too simplified.

[psilocine]

where is dicer and exportine 5 ?

[nicolaw211081]

Hmmm protein seems to degrade by enchantement after miRNA binds, ( I guess that's because we don't know yet how it works) but rather than depicting wrong ideas, maybe one should at least mention it.

[clonalmachina]

Nice work. What software do you use to make these type of movies?

[FiswaT]

Anyway I may have a copy of this video?