Gel loading and electrophoresis

[ruellan2003]

great video

[areichard2105]

@Volleyball994pimp

This was a good video, not sure what you're talking about when you say she got gel everywhere. The "gel" was already made and the "sample" is what was being loaded. I do this in my lab when conducting RT-PCR and it's not unusual to have sample expel from the well when loading, which doesn't hurt the running of the gel because it is released into the buffer.

Adam (Microbiology and Immunology Masters Program)

Boonshoft School of Medicine

Wright State University

[Milo50642]

Amazing, you beat the trolls at their own game and they stop talking? For the record, "pipping" is spelled piping or pipetting.

[volleyball994pimp]

Your way is very sloppy. like your pipping method.

[gkpeter]

@volleyball994pimp When you have time, please explain the other ways to load the gel. This actually was a student of mine and I thought it was pretty good. If there are other ways to load gels, I would be interested in knowing.

[volleyball994pimp]

@gkpeter not a diffrent way to load the gel it's just that she got the gell everywere. in my class that wouldnt be grade A work. i just think she made a mess when loading the gels.

[gkpeter]

@gkpeter Really, that is too bad you graded on something as insignificant as that. It is a dye, so there is very little DNA in there to throw off results. In real life in a real lab, that amount of "spillage" is fairly insignificant. When you load a gel with 96 samples, you don't have a lot of time to worry about "spillage". Just my two cents worth. :)

[Adz795]

Great video! Thanks alot!

[neverowned1]

i gotta do this lab tomorrow. im not quite sure what electrophoresis

[95hamsterlover]

@neverowned1 im doing it tomorrow too! . . . a year after you are (:

[gkpeter]

I do have another video that is similar and has sound. This initially was a trial.

[mikkelbakjensen]

Great video. But the sound is missing, it would have been a lot better with comments.