This procedure yields what I refer to as a "working sterile". If I'm pouring plates for undergrad labs, many times, it isn't necessary to have a 100% sterile environment in order to accomplish the task at hand. I stress that this procedure does not work if you are needing a 100% sterile plate. I recommend that if you want to pour Pyrex dishes individually when 100% sterility is not an issue, do so. That wasn't what this video was about. Thanks.
It is interesting. However, there is no mention of how he controls the depth of the agar. I see that the agar is simply poured and no indication of depth control is made. QA anyone?
I think it's more sterile if you pour them in stacks 1 by 1 so they aren't exposed to air that long. Flask in one hand and uncovering them slightly with the other. It also reduces condensation to the very top plate if you stack them.
@itsapinksnazzytie It is recommended to have 45 - 50 to have a good agar plate because agar solidifies below 45 C. It is okay to pour above 50 but it difficult to handle, other downside is more condensation that can cause problems.
@renzycute12 True, contamination via this error could occur. But it is "good enough" if you are making a large amount of plates for a casual undergraduate lab. (convienenece justifies some contaminated plates which can be discarded)
But yes, professionally, you should not pour agar like that.
Thank you so much, Dr Brumbelow. Your video is helpful. However, will it be better if you open each petri dish each time before pour in the agar ? It will minimize the exposed time of the petri dishes. Once again, thank you for the video.
I hope that will be just an weird example, agree with SarahByrne, u should use a burner or use a lab hood , any way, is a bad practice. =(
ALEXLADYDIABLA 2 weeks ago
shouldn't u be using a bunsen burner
SarahByrne 3 weeks ago
This procedure yields what I refer to as a "working sterile". If I'm pouring plates for undergrad labs, many times, it isn't necessary to have a 100% sterile environment in order to accomplish the task at hand. I stress that this procedure does not work if you are needing a 100% sterile plate. I recommend that if you want to pour Pyrex dishes individually when 100% sterility is not an issue, do so. That wasn't what this video was about. Thanks.
mbrumbelow1 1 month ago
It is interesting. However, there is no mention of how he controls the depth of the agar. I see that the agar is simply poured and no indication of depth control is made. QA anyone?
pokerace2531 4 months ago
I think it's more sterile if you pour them in stacks 1 by 1 so they aren't exposed to air that long. Flask in one hand and uncovering them slightly with the other. It also reduces condensation to the very top plate if you stack them.
RichardDawkin 10 months ago
Doh. Wear gloves and only open one plate at a time. Doing this in a hood would be even better...
jodilawson 1 year ago
why is it no less than 45oC and no more than 50oC?
itsapinksnazzytie 1 year ago
@itsapinksnazzytie It is recommended to have 45 - 50 to have a good agar plate because agar solidifies below 45 C. It is okay to pour above 50 but it difficult to handle, other downside is more condensation that can cause problems.
spk978400 5 months ago
His procedure is doubtful. He opened the plates prior to pouring of agar. There are possibilities that the plates may contaminate. I guess.
renzycute12 1 year ago
@renzycute12 True, contamination via this error could occur. But it is "good enough" if you are making a large amount of plates for a casual undergraduate lab. (convienenece justifies some contaminated plates which can be discarded)
But yes, professionally, you should not pour agar like that.
pratt123 1 year ago
im growing yeast colonies and i was wondering if the condensation on the lid would affect it at all?
HomeDistiller 2 years ago
@HomeDistiller No, it won't affect the yeast.
TwilighterTiddles 1 year ago
Thank you so much, Dr Brumbelow. Your video is helpful. However, will it be better if you open each petri dish each time before pour in the agar ? It will minimize the exposed time of the petri dishes. Once again, thank you for the video.
bocanhcam 2 years ago
this video is about prepare and pur media i think in plates, u can see and observe Dr (R)
i hope u can do
haysamrezk 2 years ago