DNA microarrays

[planetxmapevent]

The registration for Planet xMAP is now open. The European multiplexing technology symposium in Vienna (28/29 Sept '11). Visit: planetxmap[.]com

[boringgrass]

at 0:37 where did the complementary DNA come from? how did they stick there?

[arepeace0687]

@boringgrass from what im understand, the complementary strand were predesigned by the researcher based on the target sequences of the viral mRNA (or cDNA preciesely) and were initially fix to the membrane.

[haz020190]

@arepeace0687 dont the complimentary strands in total just form the whole genome of the organism? from a gene library that was available?

[arepeace0687]

'@haz020190 Yes it is. The complementary strands were account as a whole genome.

[Ubuntu3011]

Much better than my book, thanks

[kirixnaru]

thanks!!! u helped me understand microarrays right before my final

[punkyv182]

9 people suck d*cks

[adbhutseleti]

Nyc animation...helpful stuff indeed!:)

[Keirajn]

I haven't yet had the chance to use microarrays in the lab, but I need to understand this technology for a course. Can anyone explain the labeling part to me? My prof just wrote IVT-biotin labeling in the notes, how does this work? It looks to me that there is one kit that you can buy that labels your transcript, but how is there two different colored dye. Does the kit have two different dyes? Or does the two different colored dye have nothing to do with biotin labeling?

[Bblackmoonlight]

Very good. Thank you.

Medicine student, Triest, Italy.

[grybold]

Great explanation educational video. I'm a college student and this is very helpful. Thank you.

[bribrison]

This video fails to explain why your Control, lets say the green one, binds or doesn't bind to the chip, versus, the test sample, lets say the red.

The video fails to explain what is on the actual chip, that the two sample cDNA's are binding to.

[abeness88]

Great Video! We need more of this...to help understand science! Thank you!

[bloem91]

thanks, this really helped me out a lot with an asignment for school! =P

[etchedlogo]

helped me out a lot for my lab tomorrow... thanx...

[momo9476]

แปลไทยให้หน่อยได้ไหมอ่ะค่ะ

แล้วในmicroarray ใส่probe หรือ Dna เราไม่เข้าใจ

[tarlabgab]

@momo9476 ใส่ cDNA ครับ Probe แปะอยู่กับ plate

[ethanhines]

Will Rna on it's own form a loop? Or are it's nucleotides allways exposed?

[ezboy500]

Thanks a lot, you guys are great.

[TruthExposed123]

Crap !!!

[ukdori]

not clear enough

this sucks

[JanAap04]

2 tissues and each tissue fluorescent coloured differently. The damaged tissue and the healthy tissue can either act the same or differently. If it acts differently, the DNA sequence will be different, and thus not bind to certain spots where the healthy DNA can bind. Because they are fluorescent labelled, the laser can pick up the color wavelengths in the spectrum, red (Cy5, 670nm) and cy3 (green 570nm). So the colour will tell how the healthy tissue is expressed compared to the damaged tissue.

[ukdori]

i was just kidding

how can it be any easier.

thanks anyway

[slayer506]

Thank you!! I actually understand it now :)

[DonFilete]

problem with trizol is a less good purity in comparison to column purification... so u gotta analyze whats more important for ur assay 100% purity or amount of RNA .. greetz :)

[cutecloner]

nicely done !!!!!

[magiccatalyst]

holy crap. Its still pretty complicated.

[morparoh]

thanks for information to make me clear ^_^

[Leporax]

L'aiu già studiatu all'università sti cosi,su na chicchiareddra.

[aseratalgrroh]

thanx ,, i liked it so much ,, so clear :)

[dm4men]

whats the difference between an ASO array and a high density ASO(gene chip)?

[befz88]

wow, your very knowledgeable. thanks for all your help.

[befz88]

how do you know which one is infected and which wasnt if you mixed them before the array?

[Proneural]

You would typically have two different batches of cells, grown separately in culture. One batch of cells would then be infected, and after a period of time, both batches would have their RNA isolated and reverse transcribed and used for subsequent analysis.

[befz88]

And to isolate the RNA you would use phenol chloroform extraction? and then make cDNA from the RNA you collected?

[Proneural]

I use Trizol reagent to purify, which is similar to a phenol/chloroform precipitation. You just have to make sure that you eliminate genomic DNA, and DNAse digestion on RNeasy columns from Qiagen can do that. Then you can do the reverse transcription to make the cDNA.

Also, for RT-PCR, primers should be made to span an intron so that you can eliminate any possible signal from genomic DNA contamination.

[WakeUpDummies]

Exactly

[IsaacDLovesChrist]

@Proneural What is your process for designing the primers? Which company do you use to generate your RNA primers?