Make a spore syringe in 15 mins

[flashtin166]

To store spores is it better to store in the syringes or just store the prints?

[TeranTula1967]

@flashtin166 Spore prints store longer than syringes.

[modernrocks]

1Q.In treory could very few even one spore produce never ending copious myco?

also 2Q. Isnt distilled water sterile, does it need boiling?

[TeranTula1967]

@modernrocks 1A. No, not even in theory. You would need at least two spores of opposite sex to form clamp connections and exchange genetic material before any mycelium that would form mushrooms would be formed. After the mushroom is formed, then yes, an infinite supply of spores equal to the enviromental conditions provided.

2A. Distilled water is NOT sterile, and does need to be "Pressure Treated" to become fully sterile.

[AIienware]

can u mix different spore prints? and how many syringe can you filled up for 1 print?

[TeranTula1967]

@AIienware Mixing prints, yes and no. If they are the same sub-strain yes, otherwise no. Same strain/species mixes are often used to increase the viable genetic base of a culture. NOTE HOWEVER, Differing strain/species causes the mycelium to waste energy on combating other competitor species and is not advised. Typical commercial sale of a spore print syringe will have a ratio of one print to 20 or more syringes, and some companies even stretch this out to 50 syringes or more(bad). I use 1:1.

[TeranTula1967]

@AIienware Now technically, "Life Finds A Way". In some very VERY RARE cases, the two different strains will form clamp connections and actually exchange genetic material. Essentially, instead of fighting for food, they breed, essentially becoming "one mycelium patch". When I use more than one print (or portions of prints), I use the same strain but different breeding cycles, and I do this to attempt reintroduction of genetics that will hopefully assist in increasing overall viability.

[TeranTula1967]

@AIienware This is all moderated by a process called "Sectoring". Where you take a small genetic sample for culture on nutrient agar, the rest is used in actual fruiting culture. Once the Biological efficiency (how much of the food is converted into mushrooms) is determined, the culture is then evaluated for further culture. If it cultures well, then the original agar plate is noted as a "Master" for further culture, and only the smallest of amounts are ever used in mycelium expansion.

[picaticatara]

thats awesome u could get like a library of readily activating mushrooms

[TeranTula1967]

@picaticatara True. An entire culture library of spores and living mycological samples can be obtained from both commercially available strains as well as wild finds.

[harijskill]

Great video!

How long do spores remain viable after taking spore print in aluminium foil?

[TeranTula1967]

@harijskill I've found that my prints have remained viable for as much as 10 years. Though I've taken care to preserve the prints by putting them inside a double baggie, then inside a cleaned metal cookie tin and kept in a cool dark place where temperatures remain constant.

[harijskill]

@TeranTula1967 Cool! Then I don't have to worry about. Thanks.

[ordnaelcabral]

@TeranTula1967

Thanks a lot !!!Peace

[kostaz8]

i dont have a pressure cooker what should i use to sterilize the water in the syringes?

[TeranTula1967]

@kostaz8 You have a couple of options available to you.

One, you could go to the pharmacy and purchase a vial (about 10-12ml) of sterile water for about a buck and a syringe for a buck and a half, and is a fantastic way to store spore solutions.

Two, you could bring the water to a boil, wait 20 mins, then draw the water into new sterile syringes.

Though there is a practice to reuse syringes, its not really a practice I condone. $2.50 guarantees sterility success of your supplies.

[DiazH]

Thanks for the informative video.

[styrubaor]

Good video.

[nestdrumnbass]

thanks for nice instruction video. Do you think its suficient (or wise) enough to sterilize things in plastic container with alcohol soaked paper towel inside ??

[TeranTula1967]

Yes, then it was sterilized in a Pressure cooker for 60 mins at 15psi to make sure it was "sterile".

[reaper122396]

alright thank you very helpful

[brogil]

thanks for paper sterilizing tip. Question. Can you make a spore syringe from mycelium? That is all I have right now, plus a spawn bag with injection port.

[TeranTula1967]

yes indeed, though storage is more sensitive, Live culture syringes.

[parsleysprigs]

@TeranTula1967 so you said there's enough in one spore print to make 50 syringes. So if I used more than one spore print does that mean that there's less chance of contamination and the mycelium will have better resistance. And if I used the syringe right after the 24 hours of rehydration how will this improve inoculation.

[TeranTula1967]

@parsleysprigs More prnts has a greater genetic diversity for life to choose from, but not a decrease in contamination or an increase in resistances... Just a better chance at better genetics... Utilizing syringes/spores after "HYDRATION" improves germination rates and time by as much as three days.

[AlexTronic777]

Great one-handed drawing technique.

[2ManyNoobs]

how important is a glove box according to you?

I've read a lot that it doesn't matter that much, as long as you don't touch everything with your fingers it should be ok. Is that true?

[TeranTula1967]

FALSE, AND A BAD IDEA.

Do Not Dispose Of Equipment. If You Can't Afford A 5$ Glove Box From Rubber Maid, wait to cultivate.

[theonlyrealmaster]

@TeranTula1967 so you are saying that home made syringes are more powerful that the one you buy on internet plus you are saving lots of money?!? Thanks for the video are you just using ordinary green paper or is same special kind of paper?

[TeranTula1967]

@theonlyrealmaster Not more "powerful", but it does have a drastically increased chance of culture with a greater genetic diversity. Just plain green crate paper, I use tin foil now exclusivly for printing... Long story made short... at one point, I thought there might be no "green spoating mushrooms"... I've been corrected since. so now I use tinfoil.

[mitsuman95]

sweet. great step by step instructions thanks!

[zeppidy]

very well done! full of great info THANX for upping this vid!!

[TeranTula1967]

The method you used for that brown rice flour is called "pasturization", you boiled the jars for an hour. This increases the interior temprature of the jar over 170F but remaining below 200F. This essentially kills about 85% of all life that could be in there. Pressure cooking can raise that to 98%.

[TeranTula1967]

Yes, indeed, I normally wear gloves to my elbows, and In fact, the entire process should be performed in a sterile enviroment such as a glove box or flowhood. The only reason its being show outside of it, is that photography in one is someone "cramped".

[allwaystalkinshit]

Great video

[barteizzz]

how many hours can i let the liquid inside the syringe waitting until i inoculate a glass?

[TeranTula1967]

I would let them hydrate for 24 hour if the print has been in storage for a very long time. If the print is fresh, perhaps an hour.

[Souljastyle]

what is the liquid in the syringe?? water??

[TeranTula1967]

Yes. Sterile water. I pressure cooked it at 15psi for 60mins.

[limpbizquick]

way too much spores for a couple needles, you get like 10 syringes full off of ONE print

[TeranTula1967]

Indeed. Most spore providers use a ratio of 1 print to 20-50 syringes. In some cases, I use multiple prints from seperate gene pools or different genetic cycles to rejuvinate a strain.

[FFFadeToBlackKK]

Thanks alot

[maxhendrixboxer]

Cool vid, can you help me out here with a Q?

How much better is injecting the spores than simply sprinkling them? As in groth success rates.

Thanks :)

[TeranTula1967]

The reasoning behind placing spores in a liquid media is several and subtle. It can extend from easy transfer of spores to being able to maintain a sterile enviroment. Its not that "sprinkling" is not done, its jsut not the best way and since the lid of the media has to be exposed for a long time, this increases the chances of contamination where as liquid transfer is "less" likly to contaminate.

[joshcarbonera]

hey so no mushroom at all (any species including gormet) have green spores?

[TeranTula1967]

HEHE... yes, since the making of the video, I found out there are inedible and poisonus mushrooms that sporate green. So I have returned to tinfoil.

[funnyguise]

what's the minimum number of prints/syringe or syringe/print for effective inoculation?

[TeranTula1967]

This has been under some debate. In he strictest sence, you only need two unlike-sexed spores in the same media to be able to produce fruitbodies. In life and practice, at least some portion of the spores that are made by a mushroom will not germinate, the mycelium will die of various causes (natural and non). I personally use a 1:1 ratio, and ratios of 1 print to no more than 5 syringes is best for culture, and a ratio of 1:50 for mycroscopy only.

[leftxhook]

at 5:28 .......can u just put normal tap water in the syringe and put that in the pressure cooker?........in order to get the water needed to make a spore syringe?

[TeranTula1967]

This is likly to melt the plastic syringe.

PC the water first, 15psi for no less than 60mins. Then using NEW medically sterile syringes, draw the water into them while still warm/hot. Let cool before use.

[OptikNerv1138]

how long can one safely store a spore syringe without losing vitality? Also, what is optimal storage temperature? thx

optik

[TeranTula1967]

Most cultures are stored at refrigerater tempratures. Spores are stored in distilled water for long term, and its been known to work for several months to a year. Dry spore prints protected from desication can last as long as a decade.

[grymmtymm]

@TeranTula1967 what do you mean dry spores protected from desication? protected from drying out?

[pilimidian]

was all you put in there is water to seperate spores??

[TeranTula1967]

Yes. the repeasted ejection and drawing seperates the "clumps" down to spores.

[KOLIN25]

so after you used the syringes you filled them with boiling water then cool them to use them again??

[TeranTula1967]

Yes. The water is pressure cooked to steilize, then as its cooling under a flowhood, its drawn up into the syringe.

Normally. mine is too small to permit good view with the webcam at the time.

[amp244]

quick question about the sterilization process. Your just putting the jar, exacto knife, and print papers wrapped in foil, into an oven at 400 degrees F?

[TeranTula1967]

all "hard equipment" is pressure cooked at 15psi for 60mins. If it has reseted for any time, then it is wiped with alchohol and peroxide before use. Paper instructions follow... :)

[phanotm]

nice, nice, nice...

[idontfitin]

yeah, but isnt their a possibility that the paper the spores are on could have bacteria?

[TeranTula1967]

The paper was sterilized at 400F for 20 mins sandwiched in tinfoil i nthe oven and left t ocool under a flowhood.

It is sterile.

[godra051]

@TeranTula1967 Thanks man for this and now is time to do it myself......

[OriginOwns]

what kind of paper are you using/how are you sterilizing it? i know that its at a specific temperature but HOW do you do it?

[TeranTula1967]

I sandwitch the paper in some tinfoil, and bake it at 400F for at least 20mins. I use a "test paper" on the outside, that when it turns brownish, the inside paper is completly sterile.

[idontfitin]

Thank you for the video, its very informative.

[atomsanddust]

thanks

[whitegrant]

It sounds like a harddrive clicking when you are scraping the paper. Hahaha. Nice video.

[opalweiss]

This Guy Is GOOOOOOD

[BornAgainAtheist]

GREAT VIDEO.

Thanks a lot.

Is that an egg carton you've got there holding all of your print's and syringes?

Good idea if so, if not I just had a good idea!