Ok, don't do this. If you are a beginner buy a 20 cc syringe of spores online, get a glass jar fill it with a ratio of 1 tsp of karo red label corn syrup per 250 ml of water. microwave for 20 mins after that everything needs to be very sterile including yourself. use gloves and alcohol also a candle for the needle to be sterile. inject 2-8 cc's of your spores. wait a week and if you have a white cloudy jizz looking substance you probably have viable liquid culture.
I would leave the distilled/sterile water in a jar covered with a lid (one small hole inside the lid to insert the needle). the hole is covered with tape and firstly penetrated with the glowing needle (which cools immediately in the water). The lid is removed only for a few seconds to drop the spores inside the jar!
But good luck with that not nearly sterile technique.
plus it's not the best to way to 'scratch' the spores cause you can damage them that way, better to use glass or foil and wet the spores, then wash them off the glass or foil into the water....
Not BAD, but very bad technique and very unsterile procedures - sucking air into your syringe so you have a bubble??? Thats like sucking in a nice little pocket of contaminations to completely ruin your project.
However, with TWO whole prints per syringe (completely unnesecary - half a print is MORE than enough for a syringe) - the chances of contaminates getting a foothold before the mycelium grows, is greatly reduced.
He used two, I'm speculating, because half the spores more than likely went into the air around him instead of into the jar. Definitely a bad technique.
if the syringes are bought sterile than why do you need to run hot water run through it.
kealan8010 1 year ago
too Much spores,
OrganisationLudwig1 1 year ago
p.s that is not the ideal way to do this but it is a good easy method for people new to mycology.
vwiseman88 1 year ago
Ok, don't do this. If you are a beginner buy a 20 cc syringe of spores online, get a glass jar fill it with a ratio of 1 tsp of karo red label corn syrup per 250 ml of water. microwave for 20 mins after that everything needs to be very sterile including yourself. use gloves and alcohol also a candle for the needle to be sterile. inject 2-8 cc's of your spores. wait a week and if you have a white cloudy jizz looking substance you probably have viable liquid culture.
vwiseman88 1 year ago
fuking boring vid
soyagustin 2 years ago
There ARE green spores but they are not very common. Chlorophyllum molybdites A.K.A. Green spored parasol or False parasol is one example.
visschman 2 years ago
I would leave the distilled/sterile water in a jar covered with a lid (one small hole inside the lid to insert the needle). the hole is covered with tape and firstly penetrated with the glowing needle (which cools immediately in the water). The lid is removed only for a few seconds to drop the spores inside the jar!
But good luck with that not nearly sterile technique.
ataraxie85 2 years ago
couldnt you just use unopened bottled water so you dont have to boil water?
mgfurst 2 years ago
Comment removed
Flyup7 2 years ago
great tek, best one I have seen so far
rickybosskilla 2 years ago
what does he spray into the jar with the needle?
ALV57 3 years ago
cooled boiled water
shadowofhell420 3 years ago
plus it's not the best to way to 'scratch' the spores cause you can damage them that way, better to use glass or foil and wet the spores, then wash them off the glass or foil into the water....
grymmtymm 3 years ago
I bet he's doing it in front of his computer because that is where his webcam is!
CaptainMaxMushroom 4 years ago
Not BAD, but very bad technique and very unsterile procedures - sucking air into your syringe so you have a bubble??? Thats like sucking in a nice little pocket of contaminations to completely ruin your project.
However, with TWO whole prints per syringe (completely unnesecary - half a print is MORE than enough for a syringe) - the chances of contaminates getting a foothold before the mycelium grows, is greatly reduced.
OzDoofer 5 years ago 8
He used two, I'm speculating, because half the spores more than likely went into the air around him instead of into the jar. Definitely a bad technique.
rexenne 3 years ago
It's better to print your spores on tin foil to reduce the risk of paper particles getting into and possibly cloging the needle tip.
nerfaphytum 5 years ago
A glove box or flow hood is a far better method. If anything, this video is a demonstration on how not to make a spore syringe.
This guy's an idiot, i posted it to show a few buddies who r into mycology as well.
Take care guys
paradokzical 5 years ago
nice video... i just always thought a glovebox was nesscary. But if you get results ill take your word for it.
redplate 5 years ago
i dont get it whats he doing?
LoneJackel 5 years ago
thats because you are obviously not a mycologist
redplate 5 years ago
no shit but still whts he doing?
LoneJackel 5 years ago
hes taking a sporeprint from a mushroom and putting it into syringes for inoculation into a mushroom growing substrate.
redplate 5 years ago
This comment has received too many negative votes show
oh i thought he was a terrorist MUHAHAHAHAH
LoneJackel 5 years ago