Gluconeogenesis

[Safzy17]

thank you so much!

[niku0086]

its a crazy shit man,,,

whats this??????

[trixchialoveskimchi6]

this topic gives me hemorrhages ( jokes) i can barely understand my notes

[Noveleffect]

This is great for a quick review while you wait for class to start!! (2011, iPhone...life rocks lol). I know this video was created almost 3 years ago, and you've probably gone on to greener pastures with your doctorate in biochem....but Thank you, truly..these videos are awesome!

[aktorra]

thanks duDE :)

[ahmedeisa2010]

well why don't you write the molecular structure of each substance

[yvor99]

I like your explaninations of the glycolysis, and gluconeogenesis. You have made it easier to understand. Thank you:)

My hope is that you maybe could make simular presentations of the krebs cycle and the pentose phosphate pathway....?

[Kainhero]

Thank you very much for taking your time to transfer your knowledge; much appreciated.

[liveonnoevil721]

I am SO happy this is on here. It has helped me study for my final so much! THANK YOU!!!

[waqarhassanpk]

The video is not comprehensive. Teacher is not explaining anything at all, he is just proceeding with the reactions.....

Thanks anyway

[fishhujay]

thx!!!!!!!!!!!

[Sillybean28]

With the reactions he talks about starting at 3:57, they proceed in one direction. They are the reverse steps of glycolysis, but this pathway goes isn't reversible itself. It's a good video, and I thank him for taking time to do this.

[elmadarry]

when OAA (oxaloacetate) becomes PEP (phoshoenolpyruvate)using GTP, this is not a reversible reaction! great vid

[hautepink7]

Good vid. It's Phosphoenolpyruvate though. not "Phenol"

[sass1n]

@hautepink7 depends where your from

[crystal2nero]

Thankz a Million!!

[uniterscron]

nooooooooooooooo not phenol!!! phenol will kill yall. jk good vids

[heywimp]

i have a doubt man how can oxaloacetate move from mitochondria to cytosol i think it has to be first converted into malate and with the help of malate dehydrogenase it is converted again to oxaloacetae please clear my doubt

[WhiteMoon0505]

Well you are right:

oxaloacetate can't go through the mitochondrial internal membrane, so it must be reduced into malate. In the citoplasm (I'm not sure how it is said in english "citosol") malate can be retransformed in oxaloacetate, in order to proceed with gluconeogenesis. The enzime that makes possible both reactions is malato dehydrogenase (mitochondrial and citosolic respectively (is it the right term?))

[tnesty]

You guys are both right. You have to reduce OAA to Malate with NADH in the mitochondra. Malate moves out into the cytosol along with NADH which is important in inhibiting certain steps in glycolysis so that gluconeogensis can continue. Once in the cytosol Malate can be oxidized by NAD back to OAA and then to PEP by PEPCK.

[wolvereen42]

Thats straight out of the texbook!!!

Top Marks!

[gnilly1877]

So oxaloacetate is cannot go through mitochondria memebrane. it has to be converted to malate.

[alifeili]

@heywimp , yes you are right, Oxaloacetate (OAA) can not pass mitochondria. it definitely has to be converted into malate, and then in cytosol malate will be reconverted back to OAA. OAA then is converted by PEPCK using a GTP.

[sexinisrael]

@heywimp

Althought your post is 11 months old, I tell you that your assumption is correct.

It is transported by the malate-aspartate-shuttle.

[reccarebellion]

@heywimp yea, agree

[pacer10s]

@heywimp oxaloacetate(OAA) cannot move from the mitochondria to the cytosol, but that does not mean OAA is always transported to the cytoplasm via the malate shuttle. Depending on the organism, PEP carboxykinase(PEPCK) may be located strictly in the cytoplasm, strickly in the mitochon., or distributed between both. If PEPCK is present in the mitochon. then OAA can be converted to PEP which may then be shuttled out. Otherwise you are right it will move through malate or also aspartate transport

[pacer10s]

@heywimp also pyruvate carboxylase and pep carboxylkinase together under physiological conditions in the liver have a gibbs free energy around -22.6 kj/mol meaning that the reverse reaction for each is not going to happen. So the PEP carboxy kinase will only catalyze the reaction in one direction ---------> which the author has mistaken

[jisaid08]

@pacer10s I am not mistaken.

[alexandrajohnson248]

@heywimp yh you are completely right it does have to convert to malate with the aid of malate dehydrogenase to exit the mitochondria , then its re-oxidised to oxaloacetate once in the cytosol

[evidencebasedliving]

you should try this using power point and a screen recorder, i totally recmoend it, using as less lines as possible with each slide, and its gonna be much more clear and practical.

[cflores27]

you are amazinggg! =)

[koulamit]

No structures, no biochemistry! considering the time limit of ten minutes it is good, but u can do better! u dont have to write the name of the whole enzyme or the substrate, coz u r already saying it, write the short form.......... phosphoenolpyruvate (PEP) or glyceraldehyde 3 phosphate ( Gly-3-P)! Rest it was great! looking for some structural information from u...... Thanks!

[yegorovich]

Thank you very much for the video. I am evising for the model exam question assessment. Before, my biochemistry lectures would just make my brain want to shut down. But now , Gluconeogenesis, one of the major pathways is more or less clear! Will check out the glycolysis, need to revise that as well anyway.

Great work!

[mostafahahaha]

thank you:)

[LeChatlierLover]

This video is great as a review (when the student has already learned the structures and stuff) which is what I'm using it for (I have an exam in 4 days). Good job! I'm looking forward to your upcoming videos.

[kman1420]

good job man, im watching this the night before my first term med student biochem exam, btw we dont need to know the structures so this is fine. I appreciate the work.

[rocknarock88]

great job! but you can't teach biochemistry without showing the structure of the substances you use in reactions...it's pretty hard for a person 2 understand what hapens in a reaction if you don't show the the structers, so i'm waiting for your next videos...thank you very much for the explinations and i apologize for any spelling mistakes(i an romanian)!

[grigoriisdead]

phosphEnolpyruvate? is there a difference between that, and phosphoPHenol pyruvate?

[rocknarock88]

he made a mistake there...he should have written phosphoenolpyruvate

[fauzanpt]

that was quite informative..AND interesting too..thank you & keep up the good work, we appreciate it

[Treknologist]

I seriously like the idea behind these 2 videos, but the first sentence of this video is so perfectly geeky sounding that it comes off as funny. Excellent.

[jisaid08]

I aim to please haha. Glad you liked it man.

[alihassan256]

your aswom dude..woulnt mind paying u as prive tutor

[joseph0130]

@Treknologist hmmmm. Was that comment really necessary?