Enzyme-Linked Immunosorbent Assay (ELISA)

[noriharichandan]

thank you

[khijasmith]

brilliant video

[crazdiaz1]

thank you very much!

[nybigbro]

thank you. thank you thank you thank you thank you.  thank you

[Israeli6761]

I assume the test must be done quickly otherwise given enough time, the well with the more enzyme will run out of substrate and in the well with even the smallest amount of enzyme will continue catalyzing substrates and will catch up to the well with the higher amount of subrstrates

[yuchunsa]

What is the purpose of having so many wells and the dilutions?

[tessa5191]

@yuchunsa

more wells is to get more repetition for better significant results, imagine each well as a single little experiment, and dilutions help us know the different fluorescence at different dilution levels.

[piarocio2013]

@yuchunsa it's because ELISA tests can provide qualitative results, indicating whether a patient is positive or negative for the presence of the antigen or antibody, or an ELISA can provide quantitative results, determining how much of the detected substance is present. So If the ELISA test can still detect disease antigen or antibody at the smallest dilution and smallest final concentration, then the patient must have a very strong infection.

[yuchunsa]

@piarocio2013 oh.. thank you, very informative!

[saccee]

Wow!!! Thank you so much. I was anxious about ever understanding this. The animation you made made a lot of sense of what I did in lab last week.

[sunsetpeach]

thank you so much! this makes so much more sense now

[justwatchingalot]

Yeah thanks, i have a lab on this today as well and now this bit makes sense :)

[mrslloydhpolite]

Thank Yoooou.. have a lab on this tomorrow and the lab manual is long winded and didnt make sense.. now its crystal clear.. thanks :)

[Theguyinaplasticbag]

Great stuff, now I can figure it out better with that test and antigen HIV.

[melissious]

Nevermind, found it! :)

[melissious]

Fabulous! It's finally making sense! Desperately needed this for a presentation on Ebola. I've been reading up for days and watched a few videos that didn't really explain in detail what was going on. GREAT video! Now, do you happen to have one on RT-PCR?? ;)

[82Horror]

I'm studying about e coli cultured in agar ,to be clear

thnx

[82Horror]

hi guys: this film deals with serum samples but,what if I have e coli samples anybody know how e coli ELISA kits work??

thanks

[BabyArius]

Thanks lots!!! FInallyyy understood just watching once than reading it for hourss!!! =P Animations do helppp!!! Thank you!

[spldrstudtom]

thanks! Easy to understand, good explaination.

[MrMike00722]

you are a scholar and a gentleman

[snowman3475]

so confusing.you watch so many times?is that water or buffer ?why we have to used the elisa reader if the color change obviously?and it cost so much hours..

[OxAxS]

@snowman3475 Sometimes the color is not so clear and therefore you'll need to use that device. The fluid you see is serum. This test is used to check if you have antibodies against the antigen in your blood. For example used for HIV and some protozoan infections like Giardia in which fecal smear usually produces negative results.

[17Samael]

Thanks! This may well help me to pass my tests tommorow! :P

[curry12321]

thank you

[breakfast1992]

nice video.....

[ekbastu]

well done professor :).