Gel electrophoresis

[eyeslikestars001]

We are just learning about this in my AP Biology class, and this video helped me understand it a lot! Thanks! :)

[ronaldo204100]

Great video, it helped me a lot during lab!

[kyywii]

Damn i so glad i worked in a lab over the summer, otherwise i'd be screwed right about now

[CiaoBello21]

I once accidentally destroyed the agarose gel filling in my last sample, simply because i punctured the gel with the tip. Whats even funny is that i had a group of 5 others who were watching me as if their life depended on my hands.

[fappingfrenzy]

lol shaky hands

[luar2521]

thank you for this video

[Lupucillo]

Looks like Smurf jizz.

[tranceaddictallnight]

buffler @ 1:55

[volleyball994pimp]

at school we didnt make the gels we just brought them ahead of time for the lab.

[iney1000]

thanks i am doing this for science fair and i was wondering how it works

[AStrummerNamedJoe]

We did this in lab today; it can be VERY tedious.

[fcdog555]

Thank you for the video! I have to do this in college soon. It was nice finding an electrophoresis video not in spanish finally hah

[1006Will]

Hi Questions:

you made two sets of wells in a single gel, how did you know that the fragments from the upper wells won't travel THROUGH and hit the second row of wells?

secondly, how long does the whole process take?

[gkpeter]

@1006Will You can monitor it by keeping track of the migration dyes. In the video, you will see there are two colored dyes. The fastest moving one runs at about 500 base pairs. So, if you know the sizes of your DNA, you can prevent it from running into the next set of wells.

This whole thing can be done in 1.5-2 hours. Depends on your conditions and number of samples.

[BigBoss7777777]

@gkpeter Hi, I was just wondering what DNA gel is used for? Is it used in skin care products?

[canaan1967]

How are the wells numbered? 

[ZScarabello]

Hi gkpeter,

I was wondering if you or anyone else here could explain to me why there is a need to identify sizes of DNA. What is the point of this exercise? Can you extract the DNA after it has been separated?

[gkpeter]

@ZScarabello There are lots of reasons to know the sizes. Verification of constructs, genotyping, cloning, etc. But the simple answer is, yes, you can extract the DNA from the gel if needed. I have done it several times.

[HawaiiZach]

@gkpeter One reason I perform gel electrophoresis to identify the size of the DNA band is to determine if the primers I used in PCR (Polymerase chain reaction - used to amplify small quantities of DNA into the thousands) actually cut the DNA to the length I needed. If you are only focusing on looking at a certain section of DNA primers will "cut" the section out you need, then they will be amplified to the thousands using PCR. You can then run the product out on a gel to determine if it cut

[phitox008]

@ZScarabello actually not always the size is being determined. this is the way we can actually see if we have an insert (desired trait) in your DNA sample, and in this process we can cut the specific characteristic of your desired trait to put it in a transgenic species, it is important in Biotechnology, the result is called chimera. yes you can extract the DNA after separation..

[ZOMIBErevolution]

this is cool

[foeasy16]

daaaaamn this is a community college??? we dont even have these high quality molecular replication equipment and im at a university :-/

[noorchaudery]

thnx 2 upload, well plz explain the function of EtBr in electrophoresis, basically

[Peachpassion9]

how do you calculate the size of DNA fragments in the cut DNA samples?

[gkpeter]

@Peachpassion9 Compare it to a DNA ladder or molecular weight marker. You can guesstimate it or graph the known mass vs. length of migration from the well. You graph it on semi-log graph paper.

[Peachpassion9]

Do you have a video that shows this?

[Peachpassion9]

I have the lambda HindIII genome, but I don't know how I calculate the size of the DNA fragments I compare it with.

[gkpeter]

@Peachpassion9 I don't have a video for that yet, but plan on it. Maybe next week. You compare your band to the band on the marker. If they ran about the same length, then they should be about the same size.

[Peachpassion9]

@gkpeter Well, thanks for the help. I appreciate it. :)

[phitox008]

@Peachpassion9 you dont actually calculate the size, it is indicated on the first marker. note that every DNA has its own characteristics. every marker has its own interpretation.. there are application on the net that actually calculate the relatiuonships of these markers.

[jesca215]

perfect

[proteinP53]

very good

5/5

gracias por subir este video van 5 estrellas

[valkyriemissiles]

question. what is molecular weight marker? what does it do?

[kayouna]

A molecular weight marker (commonly known as the ladder) contains DNA strands with known sizes (in base pairs) and so allows you to determine the size of your samples by comparing them to that reference.

[deefromott]

Thank you.

[andythrash]

Great work there, were gonna do one next wednesday at school hope it will work!

[0749061]

Wonderful

Good work .. thanx

[amazaan]

it is handly for basic level of understanding about gel electrophoresis. thank you!!

[gkpeter]

No problem, if you need anything else explained, feel free to ask.

[sukiwooty]

can you talk a little about the results? or maybe type a few lines or something please??

cheers~

[bulletforthetrooper]

very useful. thank you

[gkpeter]

No problem, if you need anything else explained, feel free to ask.