Enzyme function and inhibition (with audio narration)

[zed2016]

woow it took me a minute to understand this compared to the 2 classes of notes i took and yet i was confused dayuuum

[wlrsruss]

The depiction of competitive inhibition is good but the non-competitive inhibition is wrong. A non-competitive inhibitor does not prevent substrate from binding, rather it prevents catalysis from occuring, so overall Vmax decreases but Km is unchanged. Also the type of Inhibition (competitive, non-competitive, mixed or uncompetitive) is defined by the kinetics NOT by how or where the inhibitor interacts with the enzyme.

[9tify1]

nice video....umm i was wondering what is irreversible inhibition then?...m guessing that the blocker attaches to the non-active site causing the enzyme to change its shape permanantely..please let me know if i am right...thnq

[CowGalz]

This is going to help me do my bio assignment ! the bitch is going to ask me for it 2morrow !

[wearesilver22]

thank you! nice voice btw ;)

[DiamondtheSimon]

Just some last minute study before my bio exam tomorrow. Cheers for the video.

[Kamillafri]

hmmm I am confused, your description of competitive inhibition soulds to me like non-competitive inhibition and your non-competitive like allosteric inhibition..

[JubbaTheHott]

@Kamillafri allosteric inhibition IS the binding of something to the non-active site of an enzyme causing a change in the shape of the enzyme's active site - which is ALSO non-competitive inhibition since it's not competing with anything for use of the actual active site. i don't know why you think competitive inhibition seems like non-competitive inhibition, though...if two things are trying to bind to the same active site, they are competing for it....

[slycordinator]

@JubbaTheHott "allosteric inhibition IS the binding of something to the non-active site of an enzyme causing a change in the shape of the enzyme's active site"

That isn't necessarily inhibitory. As far as I've read, there are some enzymes that function by one substrate attaching to an allosteric site which then causes the active site's shape to change such that it now can fit for the active substrate. :)

[vidusha123]

Short n Sweet.....thanks it helped very much.... ;)

[crizthelito]

mmmmmmmmmmmmmmmmmmmmmmmmmmm :) wiiii mi homework :)

[JubbaTheHott]

@th3don147 thanks, glad you found it useful

[TheSpinoDino]

Which programm for making this animation did you choose

[JubbaTheHott]

@TheSpinoDino I used Maya

[masterwizard155]

so what happen with a incompetitive enzyme ?

[jeydii06]

Very informative. Albeit the speaker talks too fast. I need time to process what you're saying, man. lol. Thanks for this great vid though! Best one out of five I've seen so far.

[JubbaTheHott]

@jeydii06 thanks - just watch it a few times and take notes ;)

[JubbaTheHott]

@jeydii06 thanks - i animated first and tried to fit my voice in after...next time maybe i'll slow down or loops my animation so i don't have to talk quite so fast.

[Measonz]

This video is very helpfull

[JubbaTheHott]

@Measonz thanks, glad it helped!

[SuperChelseaFans]

I have some confusion...will non-competive or competitive inhibitors stayed forever in the site? Or they just bind for few seconds and left? Please help ><... Anyway, this video really helps me on my biology final exam, Thx.

[xRosie7x]

@SuperChelseaFans It will stay there until one of the surrounding molecules (Bilions of them!) hit the enzyme and knocks it off. :)

[JSquid1337]

just a question when a non-competitive inhibitor attacks the enzyme doesn't the active site shape get altered but also substrates are still able to use it with less efficiency? that's what my teacher told me so im really confused.

[JubbaTheHott]

@JSquid1337 that is correct. this wasn't meant to be an all-encompassing animation. in some cases, the active site might not be altered enough to completely prohibit the substrate from binding.

you can also think of it like this: if there are 1000 enzymes and 500 non-competitive inhibitors present, the substrate now only has 50% of unaltered active sites available which reduces the overall efficiency by half.

[JSquid1337]

ah ok thanks! =]@JubbaTheHott

[JSquid1337]

@JubbaTheHott Ah i c thanks!

[ThePawngerardo5]

hello , can you post a download of this video I need it for my biology project thank you inadvance

[iamsowhywatch]

Ur showing something others don t understand try exposing it to common man process and not just from text who ever did the video

[ahmedmaxwell]

good video (Y) tanks 4 the help



[rhsmustangmom]

Great video... thanks for sharing!!

[MrGuugaalre]

thank you for precise explanation , i appreciated , you practical way active site ,etc ,how you makes block and color but you speech to fast , no matter thank you very much to grasped about enzymes

[JubbaTheHott]

@harena100 thanks for the suggestions - i don't intend to update this video since it was done as a quick animation proof-of-concept rather than for purely scholastic purposes, but it's good to see you know your stuff. :)

[NSZ951LIZS]

thanks a lot, seems more harder in my book than in this video of yours ;p

[JubbaTheHott]

@NSZ951LIZS you're welcome, glad it helped

[PazPanda]

Thank you! Simply perfect!

[JubbaTheHott]

@PazPanda thanks for the compliment...glad to have helped out!

[zettand]

Best video by far

[JubbaTheHott]

@zettand thanks...glad it helped!

[bebobblebobble]

did anybody ask "why?"

[JubbaTheHott]

@bebobblebobble go for it...ask away!

[kiwikidfromegypt]

@JubbaTheHott

hey thanks for the vid, i know this is a dumb question but could u tell me a lil on how enzyme activity affects cullular function. Its for an assignment and i cant find a good solid answer. Thanks for ur time.

[tjgolaso]

Makes more sense than it did before :) thanks

[srdebes]

i've been trying to figure out this section in my book for the past 30 minutes or so. this video made it easy to understand in just over a minute. awesome. thanks!

[xkuchex]

Awesome. Finally a simple, undertandable EASILLY comprehensible explanation. :)

[juzforjokez]

helped me, thanks

[thenailfamily1]

yay...thank you..make SO much more sense when you can SEE what's going on!

[b9ryan]

Nice vid, if i get an "A" 2mw its all you!

[Aqabutt426]

it's so helpful. very easy to understand. Thanks for uploading it

[JubbaTheHott]

@Aqabutt426 no problem, glad to help :)

[Aqabutt426]

@JubbaTheHott YA. THANKS SO MUCH

[zacamla]

this is going to help me so much on my bio final exam in a couple days, thank you!

[JubbaTheHott]

@zacamla you're welcome, hope the exam went well :)

[chrissline77]

@zacamla .........Did you pass the final exam for bio?

[opera1905]

Clarity makes things so much more comprehendible.

Thank you!

[JubbaTheHott]

glad to have been of assistance!

[genius4themostpart]

This was really good! So simple and the best part is that you get everything that is said!

Thank YOU!!

[RevZap8]

Well explained, great video. Very helpful. Thank you!

[JubbaTheHott]

@RevZap8 glad you liked it :)

[InLKdream]

my saviour, chem exam tomorrow took me a whole yr 2 finally learn this thank you.

[arvininfinity]

Simple, helpful and quick ..whole package which I needed :_D

[canadianchic112]

thanks. simply explained and very helpful. is it sad that i'm in AP bio and didn't understand this concept before now? yes.

[JubbaTheHott]

well I don't know what AP bio is, but at least you understand it now  :)

[sneaki602]

Great video man, makes it very clear =] ty

[JubbaTheHott]

you're welcome, glad it helped

[kurapica0]

veryy nice and helpful. thank you =] i have a request, can u please make one about transpiration pull and absorption of water through root into root cortex, please.

[JubbaTheHott]

no problem, glad to have helped!

[kittykatspain]

I agree, better than the rest :D

make more! :D

[teddyd30]

What is your background? Are you a teacher?

[JubbaTheHott]

I'm not a teacher. I have been a teaching assistant at a Canadian university, but I switched form a science career to an animation career. I figured it would be cool to combine my skills by doing something like this.

[teddyd30]

Better than all the others I've seen. Very professional-looking.

[JubbaTheHott]

Thanks, that was the goal. :)

[smstaudt]

Nicely done! May I refer to this video for a report on enzymes? The graphic just adds a whole new dimension to understanding!

[JubbaTheHott]

Sure, but just remember that this is a simplification of things and it doesn't show by-products of reactions or where energy is required, etc. So this video isn't a primary reference or anything like that. Good luck on the report.

[ogai111222333]

thanks for the helpful video

[JubbaTheHott]

you're welcome, glad to help out!

[sophlee16]

very helpful, thank you

[JubbaTheHott]

you're welcome :)