Cyclosis in Elodea

[BlueberryLee010]

thumbs up if your watching this for biology class :D or some sort of science living environment :D

[retrolovemadness]

To measure the rate of cyclosis = distance traveled/ time (s)

It would be best if you used seconds Also, try to focus on one chloroplast and track it.

[willreed37]

Why do the chloroplasts move around in the cell? Does it have to do with its function (photosynthesis)?

[aleskl]

@willreed37 Cyclosis in normal physiological conditions is important for much faster transport around the cell compared with diffusion, but is not visible like this. This kind of cyclosis seen in this video is different, because it's a defence response of the cell - the illumination under the microscope is too strong and the cell wants to hide the chloroplasts, but this ends up in fast circular movement, because the illumination is even on all sides.

[kendelol]

wow it's amazing, wonderful, i'm loving it !!!!!!!! 

[bakguteh]

saw that with a microscope yesterday.... AWESOME!!!!

[xJapFrapx]

How would you measure the rate at which the chloroplasts are moving?

[aleskl]

@xJapFrapx From this video not so easy, because the frame rate is somehow messed up. When you take a video you should know the time-point for each frame (at least in our software I think you have this information - AxioVision from Zeiss), and you have to know the spatial calibration of the image - that is how many microns are there per pixel. When you know that, you just measure the path each chloroplast travels and divide it by the time that passed between the first and last frame.

[lomascytyelchrisme]

BEAUTIFUL IMAGE

[ailene2609]

wow

[lebirammaribel54]

Would cyclosis be considered a crucial function within cells? Why?

[oneaurora]

why does it look like the only thing in these cells are chloroplasts? where is the nucleus? the vacuole?

[ajtennis25]

the other organelles are too small to be seen with this type of microscope or the microscope is on low power

[W3r0ck321]

I did this in my science class at school. The teacher said that we wouldn't be able to see the nucleus because to se it you need stain, but if we used the stain we wouldn't be able to see the chloroplasts moving because the stain would kill the plant. Hope I helped =D

[romancasco]

1 - the organelles are too small and

2 - most are colorless and need to be stained with certain chemicals..but they are certainly there :)

[guiu9878]

you need stain to see the nucleus, the chloroplasts have natural stain

[1970groupie]

Great memories! Seeing the Elodea under the microscope back in year 11 Biol with the beautiful green chloroplasts and all that surprising(to me) movement going on was very inspirational at the time. A brilliant intro to plant biology.

[sweetestsinkx]

*feeding

[sweetestsinkx]

cyclosis is a stage where slime molds are feeing on bacteria and small bits of organisms.

[timtanguay]

Actually, it isn't. Cyclosis is also known as cytoplasmic streaming, and is the result of membrane bound organelle "crawling" along the inner cell membrane. (effectively, they create a whirl pool) cyclosis is thought to be a method of moving chloroplasts into optimal light gathering positions. Amoeba use cyclosis to form their pseudopodia :) It has nothing to do with slime molds

[Isignorancebliss]

Plasmodial Slime Molds exhibit cytoplasmic streaming which my biology textbook assures me is beautiful to watch (hence my presence here) but hard to find on youtube because the stage they refer to, plasmodium, is also the genus for the parasite that causes malaria.

[rb208]

Very interesting.. What conditions are needed for cyclosis to occur?

[noobsr]

xD I was doing a experiment in class and I needed to describe the movement.

[AsianKiddo012]

same here :D

[ToAddictedToStop]

Ahh ty so much, this video just saved my lab write up on elodea lol

[whicketywack]

hey type "&fmt-18" onto the end of this video URL to watch it in high quality

[aleskl]

this doesn't work, it's still bad quality

[helloyapoop2]

its &fmt=18 not &fmt-18

[whicketywack]

yes thank you

[chris7777777777777]

God is pretty good at what he does.

[chris7777777777777]

I don't remember commenting here, haha

[saxplaya07]

what magnification was this at???

[kevinandre91]

High Power, kinda obvious if you've ever seen it.

[saxplaya07]

ughhhh duh! im a bio major i was asking for specifics......

[aleskl]

Unfortunately the movie quality here is horrible - movement of chloroplasts should be faster and smoother. More of these can be seen if you google "Time-lapse v rastlinah", however the page is not in English, but the clicking on thumbnail images should be obvious :)

[PsiClass]

Beautiful image! What kind of microscope did you use? Probably not middle school level. : D

[aleskl]

Thank you! I used a high-end research microscope from Zeiss (Axioimager Z1). The movie was recorded with 40x objective and DIC (differential interference contrast) - helps with imaging non-stained specimens.

[justbeef]

Very interesting video!