Immunofluorescence

[ApolloSkies]

Sehr gut!

AS

[karthavewillhelp]

Just so ur aware of what you have done missy...i need to tell u this...is that this video has helped me a lot! I was studying microbio and I didn't understand the difference between normal flourescent microscopy and immunofluorescence...ty very much for this vid...ur the best!!

[bored4longtimes]

I'm confused... Antibodies are huge (~150kDa) how do they get past the plasma membrane to label sub-cellular components?

Are holes poked in the membrane during fixing?

[WildForLemurs]

@bored4longtimes

You use something to 'permeabilize' the cell, like Triton X-100

[809sweety]

@bored4longtimes yes, holes are punched to allow the antibody to enter the cell. That's why one of the cons of this technique is that the sample needs to be dead. This was what my lecturer told me :)

[rottenmd]

Exelent... I will use it in my class.

[supersammie89]

I have a lab on this tomorrow - great preparation resource. Thanks !

[alifeofworship]

Extremely helpful! I did immunofluorescence microscopy on microtubules in a lab and its really hard to tell where the division of the cells are :S

[hobbez86]

Thanks! That was really helpful!