Hi - it's just that the chromophores are in the wrong respective proteins. The smaller cyclic stucture (2x rings) that's described here first as coelenterazine in Aequorin IS actually the GFP chromophore. The second structure (5x rings) described in GFP IS coelenterazine and should be in Aequorin. So just need swapping round. I think the positioning is ok and perhaps less important for visualisation. They are at the core of the proteins - protected from solvent. Check the PDB if in doubt.
Hi - it's just that the chromophores are in the wrong respective proteins. The smaller cyclic stucture (2x rings) that's described here first as coelenterazine in Aequorin IS actually the GFP chromophore. The second structure (5x rings) described in GFP IS coelenterazine and should be in Aequorin. So just need swapping round. I think the positioning is ok and perhaps less important for visualisation. They are at the core of the proteins - protected from solvent. Check the PDB if in doubt.
tjeyes 1 year ago
@tjeyes I see, thanks for the information! This animation is over a year old now, but knowing this I'm tempted to go back and fix it.
jsamsonstudios 1 year ago
Hope it's not too much trouble to fix. Keep up the excellent work!
tjeyes 1 year ago
Nice animation. However you have the chromophore structures in the wrong proteins.
Sorry to be critical - but I know my chromophores.
Thanks.
tjeyes 1 year ago
@tjeyes I'm interested, which proteins should the chromoophores be in? Are they in the wrong position in the proteins?
jsamsonstudios 1 year ago
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tjeyes 1 year ago
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tjeyes 1 year ago