F1 ATP synthase

[canadianmb9]

any1 got exam tmrow?

[yoninir]

my ATP got dried out watching this vid

[doritela2008]

The video doesnt show that the transportation of hydrogen ions into the mitochondri is what causing the ATPas to work

[ptiftgt]

I want to know if this is showing a real one or an animation

because I'm fed up with those animation ones.

[JTS11d6]

@ptiftgt A real one is too small to really see. There was an experiment by Japanese Scientists, who attacked actin filaments to the rotating part of the protein. So they could confirm that the protein rotates by seeing the much larger actin filament rotate.

[ptiftgt]

@JTS11d6 Wow! Thanks for the information.

I'm quite surprised to know that

scientists had made a theory without seeing

the real one before they proved it!

How imaginative they were!

[BlackyJerome]

but where are the Protons wich are supposed to diffuse trough the membran?

[MrSub4]

the rotation can go either way.

understanding when this happens is how world class performance is achieved.

not exactly common knowledge.

[Koepsach]

Green is ADP and blue is Pi right? But where are the protons?

[rader55555]

Ok. I think this makes sense, check the Wikipedia image for a contradiction.

[rader55555]

Nevermind about that 'rotating in the membrane part. Though I still think the rotation is in the wrong direction. Though it wouldn't be suprising that many of these animations MIGHT be wrong, as the direction was only discovered in 1998.

[rader55555]

Yes there does seem to be some things wrong with this. If you google "noji yasuda" and then click images you'll see that it should rotate countercockwise as viewed from the gamma subunit, this is doing the opposite. Also the alpha/beta subunit should rotate in vitro, you might say that the observer is in a comoving refrence frame with the alpha/beta unit, but then how does the gamma unit move in the membrane? As far as I know, it is fixed to the membrane and the alpha/beta units rotate

[xexorz]

I've always been fascinated about things like this... now with protein folding and our slow (well.. slow as compared to my lifetime) advances in genetics, am so psyched to see a model like this. Very cool video - thank you for publishing it. Now, get a kid interested in it!!!

[Boltterman37]

...BORING!

[sahkrab]

boring??

[Flux53]

This is the best ATPase video i've seen but it would be helpful to have the various components labeled somehow! Is the protein complex size proportionate with the cell membrane? It looks like it, is the production of ATP real time? what would this look like in REAL TIME... how fast does it spin, how many ATPs come flying off it per second, of course depending on conditions.. that would be nice to include..

[DrOli]

RPM is about 8 per second. 3 ATP's a turn. 24 synthesized a second if previous experiment are correct. May have changed, does sound a little slow.

[krebscycler]

The shaft can rotate both ways, depending on the electrochemical gradient. Rotating in one direction will produce ATP, and rotation in the other direction will produce ADP. This is my favorite molecule, at least for this week...

[haykier]

Isn't the shaft supposed to be rotating the other way around, in an anti-clockwise direction, watched from below (from the mitochondrial matrix)?

[F1ATPase]

No, I think the direction is OK. See another posted video of rotation based on ATP hydrolysis as viewed from the matrix:

"ATP driven rotation of the central stalk"

It is counter-clockwise. Thus, synthesis is clockwise.

[spiral91]

cool video

[kiyomixcore]

so cool

[fergmasterflex]

mmmmm energy

[GautamRangan]

awesome