@ParaglidingManiac They are some type of cyanobacteria, taken from green water at a fountain at my workplace. The magnification is VERY relative, because I use a projection technique where the eyepiece is removed and only the objective lens (100x oil immersion type) projects onto a bare CCD imager sensor. Magnification is affected greatly by distance between objective and sensor, as well as video playback resolution on your end. A good guess is more than 800x, possibly much more.
@Huffdev The way I understand it, glass optics have a resolution limit beyond which an increase in magnification only gives a larger image with more blur. Oil immersion eliminates some of that by bridging the gap between lens and slide with a glasslike fluid, thus eliminating entry and exit points for light which add to distortion. Oil is the only way to get every last bit of performance out of a glass lens microscope.
@Navid115 Thanks! I need to get off my a**, maybe in the next couple of days, and do another microscope vid.. It's been a long time since I've done anything with it!
Thanks Ryanator! And yeah, I didn't either. Truth be told, I was a bit shocked when I saw the results. BTW... I now know that the monitor view (I usually work at or close to full screen) is about 4 times the apparent magnification of the eyepiece view... Scary, huh? That means at 1600x eyepiece mag, I am at around 6400x on screen. Mind you, it's FAR from *that* useful mag due to lack of sharpness, but still omg... it's GREAT.
@NightRunner417 wait so when you look through the microscope you don't see it like that? I'm about to buy a variscope with 2000x zoom trinocular. Would i need like a 60x eyepiece(x100) to see it like that?
@ArielCaboob Yep, that's right. The act of projecting the objective image directly onto the camera sensor inflates the magnification considerably, and then blowing it up onto a big monitor screen probably doubles it again. The result is MUCH higher magnification than the scope is rated for. To get equivalent mags, you'd need a very high power eyepiece, and then you'll be battling illumination and heat. For these and many other reasons, camera projection imaging is definitely superior.
@ArielCaboob Just to make sure you understand, you remove the eyepiece, remove the camera's lens, and place it over the empty eyepiece tube. I should think that with a trinocular, you simply can choose from three eyepieces to remove vs. my one. Remember, any time light is split by any means, the final light power is divided. Thus, my monocular scope has more light to work at the one eyepiece with than a binocular or trinocular would at each eyepiece, very important when working with a cam.
hi, i just bought an amscope off of ebay, and i am having major problems using the 100x high power. i dont really understand how to use it, ive lubricated the small lense with it but it just made things more blurry, please help.
Make sure you are sandwiching the drop between the cover slip and the objective lens tip - it MUST be touching both at the same time. Also, be careful of your focus distance, because at powers this high, the working distance is TINY. What model Amscope microscope did you get?
What type of bacteria are these and what magnification did you use to make this video?
ParaglidingManiac 7 months ago
@ParaglidingManiac They are some type of cyanobacteria, taken from green water at a fountain at my workplace. The magnification is VERY relative, because I use a projection technique where the eyepiece is removed and only the objective lens (100x oil immersion type) projects onto a bare CCD imager sensor. Magnification is affected greatly by distance between objective and sensor, as well as video playback resolution on your end. A good guess is more than 800x, possibly much more.
NightRunner417 7 months ago
What makes oil emmersion better
Huffdev 10 months ago
@Huffdev The way I understand it, glass optics have a resolution limit beyond which an increase in magnification only gives a larger image with more blur. Oil immersion eliminates some of that by bridging the gap between lens and slide with a glasslike fluid, thus eliminating entry and exit points for light which add to distortion. Oil is the only way to get every last bit of performance out of a glass lens microscope.
NightRunner417 10 months ago
u sound like u hate ur life.
MrBrownns 1 year ago
@MrBrownns You type like a Youtube psychoanalyst.
NightRunner417 1 year ago 7
Awesome work! Very helpful
Navid115 1 year ago
@Navid115 Thanks! I need to get off my a**, maybe in the next couple of days, and do another microscope vid.. It's been a long time since I've done anything with it!
NightRunner417 1 year ago
Nice demonstration! I didn't know such quality was available at this kind of magnification.
RyanatorML2000 2 years ago
Thanks Ryanator! And yeah, I didn't either. Truth be told, I was a bit shocked when I saw the results. BTW... I now know that the monitor view (I usually work at or close to full screen) is about 4 times the apparent magnification of the eyepiece view... Scary, huh? That means at 1600x eyepiece mag, I am at around 6400x on screen. Mind you, it's FAR from *that* useful mag due to lack of sharpness, but still omg... it's GREAT.
NightRunner417 2 years ago
@NightRunner417 wait so when you look through the microscope you don't see it like that? I'm about to buy a variscope with 2000x zoom trinocular. Would i need like a 60x eyepiece(x100) to see it like that?
ArielCaboob 1 year ago
@ArielCaboob Yep, that's right. The act of projecting the objective image directly onto the camera sensor inflates the magnification considerably, and then blowing it up onto a big monitor screen probably doubles it again. The result is MUCH higher magnification than the scope is rated for. To get equivalent mags, you'd need a very high power eyepiece, and then you'll be battling illumination and heat. For these and many other reasons, camera projection imaging is definitely superior.
NightRunner417 1 year ago
@NightRunner417 so you put it over the eye piece. What about those that have the trinocular one, is that the same than putting it over the eye piece?
ArielCaboob 1 year ago
@ArielCaboob Just to make sure you understand, you remove the eyepiece, remove the camera's lens, and place it over the empty eyepiece tube. I should think that with a trinocular, you simply can choose from three eyepieces to remove vs. my one. Remember, any time light is split by any means, the final light power is divided. Thus, my monocular scope has more light to work at the one eyepiece with than a binocular or trinocular would at each eyepiece, very important when working with a cam.
NightRunner417 1 year ago
hi, i just bought an amscope off of ebay, and i am having major problems using the 100x high power. i dont really understand how to use it, ive lubricated the small lense with it but it just made things more blurry, please help.
poostew 2 years ago
Make sure you are sandwiching the drop between the cover slip and the objective lens tip - it MUST be touching both at the same time. Also, be careful of your focus distance, because at powers this high, the working distance is TINY. What model Amscope microscope did you get?
NightRunner417 2 years ago